Journal ArticleDOI
HPLC method for evaluation of the free radical-scavenging activity of foods by using 1,1-diphenyl-2-picrylhydrazyl
TLDR
An HPLC method using 1,1-diphenyl-2-picrylhydrazyl (DPPH) determined the free radical-scavenging activity of several antioxidants: ascorbic acid, alpha-tocopherol, Trolox, and cysteine, and the results well agree with those of previous reports.Abstract:
An HPLC method for evaluation of the free radical-scavenging activity of foods by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) is reported. The activity was evaluated by measuring the decrease of DPPH detected at 517 nm. By using this novel method, we determined the free radical-scavenging activity of several antioxidants: ascorbic acid, α-tocopherol, Trolox, and cysteine. The results gave good correlation between the radical-scavenging activity determined by HPLC and by conventional colorimetry. This methodology was applied to determine the free radical-scavenging activity of 8 beverages. The activity of coffee was the highest, followed by red wine, green tea, oolong tea, black tea, rose wine, white wine, and orange juice. The results well agree with those of previous reports. This method is expected to be useful for a simple and rapid determination of free radical-scavenging activity in colored foods, because coloring substances in foods do not interfere with the measurement.read more
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Antioxidant and antiacetylcholinesterase activities of essential oils from Cymbopogon schoenanthus L. Spreng. Determination of chemical composition by GC–mass spectrometry and 13C NMR
Ayda Khadri,Maria Luísa Serralheiro,José M.F. Nogueira,Mohamed Neffati,Samira Smiti,Maria Eduarda M. Araújo +5 more
TL;DR: In this paper, the chemical composition, antioxidant activities and acetylcholinesterase inhibitory properties of the essential oils from fresh leaves, dried leaves and roots collected from three different locations in southern Tunisia, were evaluated.
Journal ArticleDOI
Chemical composition and antioxidant activity of yerba-mate (Ilex paraguariensis A.St.-Hil., Aquifoliaceae) extract as obtained by spray drying.
Kleber Alves Santos Berté,Marcia R. Beux,Patrícia Kelly Wilmsen Dalla Santa Spada,Mirian Salvador,Rosemary Hoffmann-Ribani +4 more
TL;DR: The yerba-mate spray-dried extract presents high catalase-like activity, suggesting that it is a strong free-radical scavenger and the antioxidant activity was related to total polyphenol content.
Journal ArticleDOI
Isolation of ellagic acid from the aqueous extract of the roots of Decalepis hamiltonii: antioxidant activity and cytoprotective effect.
TL;DR: The ellagic acid (EA) as mentioned in this paper is a new addition to the list of antioxidant compounds in the roots of Decalepis hamiltonii and has shown free radical-scavenging activity, inhibited LDL oxidation and showed cytoprotective effect against xenobiotic-induced oxidative stress in Ehrlich Ascites tumor cells.
Journal ArticleDOI
Antihemolytic and antioxidant activities of Allium paradoxum
TL;DR: Antioxidant activity of the aerial part and bulbs of Allium paradoxum was investigated by eight in vitro assay systems as discussed by the authors, and the results showed that the extracts showed good antioxidant activity.
Journal ArticleDOI
Antioxidant properties of different molecular weight polysaccharides from Athyrium multidentatum (Doll.) Ching
Jiwen Sheng,Yanlong Sun +1 more
TL;DR: All CPA derivatives showed pronounced antioxidant activity, and had stronger antioxidant ability than CPA in certain tests, and the mechanism on influence the antioxidant activity of CPA and its degraded derivatives was indicated.
References
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Journal ArticleDOI
Antioxidant Determinations by the Use of a Stable Free Radical
TL;DR: In this paper, the chemical nature of the antioxidant is known and a test specific for the compound or group of interest; for example, the nitroprusside test for sulphydryl groups.
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Dietary antioxidant flavonoids and risk of coronary heart disease: the Zutphen Elderly Study.
TL;DR: Flavonoids in regularly consumed foods may reduce the risk of death from coronary heart disease in elderly men and showed an inverse relation with incidence of myocardial infarction.
Journal ArticleDOI
Wine, alcohol, platelets, and the French paradox for coronary heart disease
Serge Renaud,M de Lorgeril +1 more
TL;DR: Data from Caerphilly, Wales, show that platelet aggregation, which is related to CHD, is inhibited significantly by alcohol at levels of intake associated with reduced risk of CHD.
Journal ArticleDOI
The occurrence of superoxide anion in the reaction of reduced phenazine methosulfate and molecular oxygen
TL;DR: The reduction of nitro blue tetrazolium (NitroBT) with NADH mediated by phenazine methosulfate (PMS) under aerobic conditions was inhibited upon addition ofsuperoxide dismutase, indicating the involvement of superoxide aninon radical in the reduction of NitroBT.
Journal ArticleDOI
Antioxidant activity of various tea extracts in relation to their antimutagenicity
Gow-Chin Yen,Hui-Yin Chen +1 more
TL;DR: The relationship between antioxidant activity and antimutagenicity of various tea extracts (green tea, pouchong tea, oolong tea and black tea) was investigated in this article, which showed that all tea extracts exhibited markedly antioxidant activity.