Journal ArticleDOI
HPLC method for evaluation of the free radical-scavenging activity of foods by using 1,1-diphenyl-2-picrylhydrazyl
TLDR
An HPLC method using 1,1-diphenyl-2-picrylhydrazyl (DPPH) determined the free radical-scavenging activity of several antioxidants: ascorbic acid, alpha-tocopherol, Trolox, and cysteine, and the results well agree with those of previous reports.Abstract:
An HPLC method for evaluation of the free radical-scavenging activity of foods by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) is reported. The activity was evaluated by measuring the decrease of DPPH detected at 517 nm. By using this novel method, we determined the free radical-scavenging activity of several antioxidants: ascorbic acid, α-tocopherol, Trolox, and cysteine. The results gave good correlation between the radical-scavenging activity determined by HPLC and by conventional colorimetry. This methodology was applied to determine the free radical-scavenging activity of 8 beverages. The activity of coffee was the highest, followed by red wine, green tea, oolong tea, black tea, rose wine, white wine, and orange juice. The results well agree with those of previous reports. This method is expected to be useful for a simple and rapid determination of free radical-scavenging activity in colored foods, because coloring substances in foods do not interfere with the measurement.read more
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Pharmacological importance of sulphated polysaccharide carrageenan from red seaweed Kappaphycus alvarezii in comparison with commercial carrageenan.
Arumugampillai Manimehalai Suganya,Muthusamy Sanjivkumar,Manohar Navin Chandran,Arunachalam Palavesam,Grasian Immanuel +4 more
TL;DR: Results indicated the beneficial effect of native and commercial carrageenans as anticancer agents being a free radical scavenger and anti-diabetic property of both Carrageenan showed inhibition effect on α- glucosidase enzyme.
Journal ArticleDOI
Screening of the antioxidant, antimicrobial and DNA damage protection potentials of the aqueous extract of Asplenium ceterach DC.
TL;DR: In this paper, in vitro antioxidant, antimicrobial and DNA damage protection potentials of the aqueous extract of Asplenium ceterach was firstly evaluated in addition to its total phenolic and flavonoid contents.
Journal ArticleDOI
Antioxidant properties of cold and hot water extracts of cocoa, Hibiscus flower extract, and ginger beverage blends
TL;DR: In this paper, the cold and hot water extracts of the beverages contained total phenolic content ranging from 11.61 to 22.01 µg GAE/g; the ferric reducing property ranged from 10.84 to 26.88 µg; and the vitamin C content ranged from 1.77 to 4.82 µg/g.
Journal ArticleDOI
Effect of Centella asiatica leaf powder on oxidative markers in brain regions of prepubertal mice in vivo and its in vitro efficacy to ameliorate 3-NPA-induced oxidative stress in mitochondria.
George K. Shinomol,Muralidhara +1 more
TL;DR: It is suggested that CA has the propensity to modulate both endogenous and neurotoxicant induced oxidative impairments in the brain and may be effectively employed as a neuroprotective adjuvant to abrogate oxidative stress in vivo.
Journal ArticleDOI
Antibacterial Activity and the Variation of Tanacetum parthenium (L.) Schultz Bip. Essential Oils from Turkey
TL;DR: Water-distilled essential oils from herbal parts of Tanacetum parthenium from two different localities in Turkey showed toxicity to Vibrio fischeri in the TLC-bioluminescence assay and antibacterial activity was evaluated for five Gram (+) and five Gram (-) bacteria by using a broth microdilution assay.
References
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Journal ArticleDOI
Antioxidant Determinations by the Use of a Stable Free Radical
TL;DR: In this paper, the chemical nature of the antioxidant is known and a test specific for the compound or group of interest; for example, the nitroprusside test for sulphydryl groups.
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Dietary antioxidant flavonoids and risk of coronary heart disease: the Zutphen Elderly Study.
TL;DR: Flavonoids in regularly consumed foods may reduce the risk of death from coronary heart disease in elderly men and showed an inverse relation with incidence of myocardial infarction.
Journal ArticleDOI
Wine, alcohol, platelets, and the French paradox for coronary heart disease
Serge Renaud,M de Lorgeril +1 more
TL;DR: Data from Caerphilly, Wales, show that platelet aggregation, which is related to CHD, is inhibited significantly by alcohol at levels of intake associated with reduced risk of CHD.
Journal ArticleDOI
The occurrence of superoxide anion in the reaction of reduced phenazine methosulfate and molecular oxygen
TL;DR: The reduction of nitro blue tetrazolium (NitroBT) with NADH mediated by phenazine methosulfate (PMS) under aerobic conditions was inhibited upon addition ofsuperoxide dismutase, indicating the involvement of superoxide aninon radical in the reduction of NitroBT.
Journal ArticleDOI
Antioxidant activity of various tea extracts in relation to their antimutagenicity
Gow-Chin Yen,Hui-Yin Chen +1 more
TL;DR: The relationship between antioxidant activity and antimutagenicity of various tea extracts (green tea, pouchong tea, oolong tea and black tea) was investigated in this article, which showed that all tea extracts exhibited markedly antioxidant activity.