Mediator and RNA polymerase II clusters associate in transcription-dependent condensates
Won-Ki Cho,Jan-Hendrik Spille,Micca Hecht,Choongman Lee,Charles H. Li,Valentin Grube,Valentin Grube,Ibrahim I Cisse +7 more
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TLDR
This work used live-cell superresolution and light-sheet imaging to study the organization and dynamics of the Mediator coactivator and RNA polymerase II (Pol II) directly and suggests that large clusters of Mediator, recruited by transcription factors at large or clustered enhancer elements, interact with large Pol II clusters in transcriptional condensates in vivo.Abstract:
Models of gene control have emerged from genetic and biochemical studies, with limited consideration of the spatial organization and dynamics of key components in living cells. We used live-cell superresolution and light-sheet imaging to study the organization and dynamics of the Mediator coactivator and RNA polymerase II (Pol II) directly. Mediator and Pol II each form small transient and large stable clusters in living embryonic stem cells. Mediator and Pol II are colocalized in the stable clusters, which associate with chromatin, have properties of phase-separated condensates, and are sensitive to transcriptional inhibitors. We suggest that large clusters of Mediator, recruited by transcription factors at large or clustered enhancer elements, interact with large Pol II clusters in transcriptional condensates in vivo.read more
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Phase separation drives the self-assembly of mitochondrial nucleoids for transcriptional modulation.
Qi Long,Yanshuang Zhou,Hao Wu,Shiwei Du,Shiwei Du,Mingli Hu,Mingli Hu,Juntao Qi,Juntao Qi,Wei Li,Wei Li,Jingyi Guo,Yi Wu,Liang Yang,Ge Xiang,Liang Wang,Shouhua Ye,Jiayuan Wen,Heng Mao,Junwei Wang,Hui Zhao,Wai-Yee Chan,Jinsong Liu,Yonglong Chen,Pilong Li,Xingguo Liu,Xingguo Liu +26 more
TL;DR: In this article, a model of mitochondrial nucleoid self-assembly driven by phase separation, and a pattern of co-phase separation involved in mitochondrial transcriptional regulation, was proposed, which orchestrates the roles of TFAM in both mtDNA organization and transcription.
Journal ArticleDOI
Dynamics of transcriptional enhancers and chromosome topology in gene regulation
Moe Yokoshi,Takashi Fukaya +1 more
TL;DR: It is proposed that assembly of “transcription hubs” in the context of 3D genome structure plays an important role in transcriptional regulation.
Posted Content
Bridging-induced microphase separation: photobleaching experiments, chromatin domains, and the need for active reactions
TL;DR: The mechanism and consequences of the 'bridging-induced attraction' are reviewed, a generic biophysical principle that underpins some existing models for chromosome organization in 3D and provides an explanation for the biogenesis of nuclear bodies and transcription factories via microphase separation.
Journal ArticleDOI
Nuclear Condensates of p300 Formed Though the Structured Catalytic Core Can Act as a Storage Pool of p300 with Reduced HAT Activity
Yi Zhang,Kyle Brown,Yucong Yu,Ziad Ibrahim,Mohamad Zandian,Steven Ingersoll,Thomas Lee,Christopher C. Ebmeier,Jiuyang Liu,Daniel Panne,Xiaobing Shi,Xiaojun Ren,Tatiana G. Kutateladze +12 more
TL;DR: A model in which p300 condensates can act as a storage pool of the protein with reduced HAT activity, allowing p300 to be compartmentalized and concentrated at poised or repressed chromatin regions is suggested.
Journal ArticleDOI
Liquid demixing in elastic networks: Cavitation, permeation, or size selection?
TL;DR: In this article , the trade-offs between elastic, wetting, and interfacial energies of phase-separated liquid condensates interact mechanically with surrounding elastic networks such as chromatin and cytoskeleton.
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