Mediator and RNA polymerase II clusters associate in transcription-dependent condensates
Won-Ki Cho,Jan-Hendrik Spille,Micca Hecht,Choongman Lee,Charles H. Li,Valentin Grube,Valentin Grube,Ibrahim I Cisse +7 more
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TLDR
This work used live-cell superresolution and light-sheet imaging to study the organization and dynamics of the Mediator coactivator and RNA polymerase II (Pol II) directly and suggests that large clusters of Mediator, recruited by transcription factors at large or clustered enhancer elements, interact with large Pol II clusters in transcriptional condensates in vivo.Abstract:
Models of gene control have emerged from genetic and biochemical studies, with limited consideration of the spatial organization and dynamics of key components in living cells. We used live-cell superresolution and light-sheet imaging to study the organization and dynamics of the Mediator coactivator and RNA polymerase II (Pol II) directly. Mediator and Pol II each form small transient and large stable clusters in living embryonic stem cells. Mediator and Pol II are colocalized in the stable clusters, which associate with chromatin, have properties of phase-separated condensates, and are sensitive to transcriptional inhibitors. We suggest that large clusters of Mediator, recruited by transcription factors at large or clustered enhancer elements, interact with large Pol II clusters in transcriptional condensates in vivo.read more
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Advances in Chromatin and Chromosome Research: Perspectives from Multiple Fields
Andrews Akwasi Agbleke,Assaf Amitai,Jason D. Buenrostro,Aditi Chakrabarti,Lingluo Chu,Anders S. Hansen,Kristen M. Koenig,Ajay S. Labade,Sirui Liu,Tadasu Nozaki,Sergey Ovchinnikov,Andrew Seeber,Haitham A. Shaban,Jan-Hendrik Spille,Andrew D. Stephens,Jun-Han Su,Dushan N. Wadduwage +16 more
TL;DR: Technologies that will be crucial to interrogate key questions in chromatin and chromosome biology are shone a spotlight on including state-of-the-art microscopy techniques, tools to physically manipulate chromatin, single-cell methods to measure chromatin accessibility, computational imaging with neural networks and analytical tools to interpret chromatin structure and dynamics.
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ALS and FTD: Where RNA metabolism meets protein quality control.
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Spirits in the Material World: Enhancer RNAs in Transcriptional Regulation
Tim Y. Hou,W. Lee Kraus +1 more
TL;DR: Roles for eRNAs in enhancer-promoter looping, recruiting transcriptional machinery, and facilitating RNA polymerase pause-release to regulate gene expression are revealed.
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Inositol Polyphosphate Multikinase Inhibits Liquid-Liquid Phase Separation of TFEB to Negatively Regulate Autophagy Activity.
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Deconstructing virus condensation.
Nora Lopez,Gabriela Camporeale,Mariano Salgueiro,Silvia S. Borkosky,Araceli Visentin,Ramon Peralta-Martinez,María Eugenia Loureiro,Gonzalo de Prat-Gay +7 more
TL;DR: In this paper, the basic principles of liquid-liquid phase separation are discussed in connection with several examples of VFs and a view which integrates viral replication mechanisms with the biochemistry underlying liquid-like organelles is proposed.
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