New world bats harbor diverse influenza A viruses.
Suxiang Tong,Xueyong Zhu,Yan Li,Mang Shi,Jing Zhang,Melissa Bourgeois,Hua Yang,Xianfeng Chen,Sergio Recuenco,Jorge Gomez,Li-Mei Chen,Adam Johnson,Ying Tao,C. Dreyfus,Wenli Yu,Ryan McBride,Paul J. Carney,Amy T. Gilbert,Jessie Chang,Zhu Guo,Charles T. Davis,James C. Paulson,James Stevens,Charles E. Rupprecht,Charles E. Rupprecht,Edward C. Holmes,Edward C. Holmes,Ian A. Wilson,Ruben O. Donis +28 more
Reads0
Chats0
TLDR
Using consensus degenerate RT-PCR, a novel influenza A virus is identified in a flat-faced fruit bat from Peru, indicating that bats constitute a potentially important and likely ancient reservoir for a diverse pool of influenza viruses.Abstract:
Aquatic birds harbor diverse influenza A viruses and are a major viral reservoir in nature. The recent discovery of influenza viruses of a new H17N10 subtype in Central American fruit bats suggests that other New World species may similarly carry divergent influenza viruses. Using consensus degenerate RT-PCR, we identified a novel influenza A virus, designated as H18N11, in a flat-faced fruit bat (Artibeus planirostris) from Peru. Serologic studies with the recombinant H18 protein indicated that several Peruvian bat species were infected by this virus. Phylogenetic analyses demonstrate that, in some gene segments, New World bats harbor more influenza virus genetic diversity than all other mammalian and avian species combined, indicative of a long-standing host-virus association. Structural and functional analyses of the hemagglutinin and neuraminidase indicate that sialic acid is not a ligand for virus attachment nor a substrate for release, suggesting a unique mode of influenza A virus attachment and activation of membrane fusion for entry into host cells. Taken together, these findings indicate that bats constitute a potentially important and likely ancient reservoir for a diverse pool of influenza viruses.read more
Citations
More filters
Journal ArticleDOI
Viral Zoonoses That Fly with Bats: A Review
Alfonso Calderón,Camilo Guzmán,Jorge Salazar-Bravo,Luiz Tadeu Moraes Figueiredo,Salim Mattar,Germán Arrieta +5 more
TL;DR: A summary of the potential societal (economic and epidemiological) effect of disease outbreaks of virus families associated with bats, and the preventive and control measures that could be anticipated are presented.
Multitask Learning of Signaling and Regulatory Networks with Application to Studying Human Response to Flu
TL;DR: In this article, a multi-task learning method was proposed to reconstruct dynamic human response networks for three flu strains: H1N1, H5N1 and H3N2.
Journal ArticleDOI
Evaluation of a primary course of H9N2 vaccine with or without AS03 adjuvant in adults: A phase I/II randomized trial.
Anuradha Madan,Harry Collins,Eric Sheldon,Louise Frenette,Laurence Chu,Damien Friel,Mamadou Dramé,David W. Vaughn,Bruce L. Innis,Anne Schuind +9 more
TL;DR: All H9N2 vaccine formulations were immunogenic with a clinically acceptable safety profile; adjuvanted formulations were 4-8 times dose-sparing (3.75-1.9vs 15µgHA).
Journal ArticleDOI
Survival analysis of infected mice reveals pathogenic variations in the genome of avian H1N1 viruses
Zeynep A. Koçer,Yiping Fan,Robert Huether,John C. Obenauer,Richard J. Webby,Jinghui Zhang,Robert G. Webster,Gang Wu +7 more
TL;DR: This study introduces a new approach to identify pathogenic variations in wild-type viruses circulating in the natural reservoirs and ultimately to understand their infectious risks to humans as part of risk assessment efforts towards the emergence of future pandemic strains.
Journal ArticleDOI
Evaluating influenza vaccines: progress and perspectives
TL;DR: This review outlines the promising steps that have been taken toward the development of a broadly protective influenza virus vaccine through the use of new technologies.
References
More filters
Journal ArticleDOI
MEGA5: Molecular Evolutionary Genetics Analysis using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods
Koichiro Tamura,Daniel S. Peterson,Nicholas Peterson,Glen Stecher,Masatoshi Nei,Sudhir Kumar +5 more
TL;DR: The newest addition in MEGA5 is a collection of maximum likelihood (ML) analyses for inferring evolutionary trees, selecting best-fit substitution models, inferring ancestral states and sequences, and estimating evolutionary rates site-by-site.
Book ChapterDOI
Processing of X-ray diffraction data collected in oscillation mode
Zbyszek Otwinowski,Wladek Minor +1 more
TL;DR: The methods presented in the chapter have been applied to solve a large variety of problems, from inorganic molecules with 5 A unit cell to rotavirus of 700 A diameters crystallized in 700 × 1000 × 1400 A cell.
Journal ArticleDOI
Coot: model-building tools for molecular graphics.
Paul Emsley,Kevin Cowtan +1 more
TL;DR: CCP4mg is a project that aims to provide a general-purpose tool for structural biologists, providing tools for X-ray structure solution, structure comparison and analysis, and publication-quality graphics.
Journal ArticleDOI
PHENIX: a comprehensive Python-based system for macromolecular structure solution
Paul D. Adams,Paul D. Adams,Pavel V. Afonine,Gábor Bunkóczi,Vincent B. Chen,Ian W. Davis,Nathaniel Echols,Jeffrey J. Headd,Li-Wei Hung,Gary J. Kapral,Ralf W. Grosse-Kunstleve,Airlie J. McCoy,Nigel W. Moriarty,Robert D. Oeffner,Randy J. Read,David S. Richardson,Jane S. Richardson,Thomas C. Terwilliger,Peter H. Zwart +18 more
TL;DR: The PHENIX software for macromolecular structure determination is described and its uses and benefits are described.
Journal ArticleDOI
Refinement of macromolecular structures by the maximum-likelihood method.
TL;DR: The likelihood function for macromolecular structures is extended to include prior phase information and experimental standard uncertainties and the results derived are consistently better than those obtained from least-squares refinement.
Related Papers (5)
Human Infection with a Novel Avian-Origin Influenza A (H7N9) Virus
Rongbao Gao,Bin Cao,Yunwen Hu,Zijian Feng,Dayan Wang,Wanfu Hu,Jian Chen,Zhijun Jie,Haibo Qiu,Ke Xu,Xuewei Xu,Hongzhou Lu,Wenfei Zhu,Zhancheng Gao,Nijuan Xiang,Yinzhong Shen,Zebao He,Y. Gu,Zhiyong Zhang,Yi Yang,Xiang Zhao,Lei Zhou,Xiaodan Li,Shumei Zou,Ye Zhang,Xiyan Li,Lei Yang,Junfeng Guo,Jie Dong,Qun Li,Libo Dong,Yun Zhu,Tian Bai,Shiwen Wang,Pei Hao,Weizhong Yang,Yanping Zhang,Jun Han,Hongjie Yu,Dexin Li,George F. Gao,Guizhen Wu,Yu Wang,Zhenghong Yuan,Yuelong Shu +44 more
Antigenic and Genetic Characteristics of Swine-Origin 2009 A(H1N1) Influenza Viruses Circulating in Humans
Rebecca Garten,C. Todd Davis,Colin A. Russell,Colin A. Russell,Bo Shu,Stephen Lindstrom,Amanda Balish,Wendy Sessions,Xiyan Xu,Eugene Skepner,Varough M. Deyde,Margaret Okomo-Adhiambo,Larisa V. Gubareva,John R. Barnes,Catherine B. Smith,Shannon L. Emery,Michael J. Hillman,Pierre Rivailler,James A. Smagala,Miranda de Graaf,Miranda de Graaf,David F. Burke,Ron A. M. Fouchier,Claudia Pappas,Celia Alpuche-Aranda,Hugo López-Gatell,Hiram Olivera,Irma Lopez,Christopher A. Myers,Dennis J. Faix,Patrick J. Blair,Cindy Yu,Kimberly M. Keene,P. David Dotson,David Boxrud,Anthony R. Sambol,Syed H. Abid,Kirsten St. George,Tammy L. Bannerman,Amanda L. Moore,David J. Stringer,Patricia Blevins,Gail J. Demmler-Harrison,Michele Ginsberg,Paula Kriner,Steve Waterman,Sandra Smole,Hugo Guevara,Edward A. Belongia,Patricia A. Clark,Sara T. Beatrice,Ruben O. Donis,Jacqueline M. Katz,Lyn Finelli,Carolyn B. Bridges,Michael W. Shaw,Daniel B. Jernigan,Timothy M. Uyeki,Derek J. Smith,Derek J. Smith,Derek J. Smith,Alexander Klimov,Nancy J. Cox +62 more