PIVlab – Towards User-friendly, Affordable and Accurate Digital Particle Image Velocimetry in MATLAB
William Thielicke,Eize Stamhuis +1 more
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TLDR
The accuracy of several algorithms was determined and the best performing methods were implemented in a user-friendly open-source tool for performing DPIV flow analysis in Matlab.Abstract:
Digital particle image velocimetry (DPIV) is a non-intrusive analysis technique that is very popular for mapping flows quantitatively. To get accurate results, in particular in complex flow fields, a number of challenges have to be faced and solved: The quality of the flow measurements is affected by computational details such as image pre-conditioning, sub-pixel peak estimators, data validation procedures, interpolation algorithms and smoothing methods. The accuracy of several algorithms was determined and the best performing methods were implemented in a user-friendly open-source tool for performing DPIV flow analysis in Matlab.read more
Citations
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Theory of cross-correlation analysis of PIV images : Image analysis as measuring technique in flows
R. D. Keane,R. J. Adrian +1 more
TL;DR: In this article, cross-correlation methods of interrogation of successive single-exposure frames can be used to measure the separation of pairs of particle images between successive frames, which can be optimized in terms of spatial resolution, detection rate, accuracy and reliability.
Journal ArticleDOI
A four-organ-chip for interconnected long-term co-culture of human intestine, liver, skin and kidney equivalents
Ilka Maschmeyer,Alexandra Lorenz,Katharina Schimek,Tobias Hasenberg,Anja Patricia Ramme,Juliane Hübner,Marcus Lindner,Christopher Drewell,Sophie Bauer,Alexander Thomas,Naomia Sisoli Sambo,Frank Sonntag,Roland Lauster,Uwe Marx +13 more
TL;DR: This study is the first approach to establish a system for in vitro microfluidic ADME profiling and repeated dose systemic toxicity testing of drug candidates over 28 days in co-culture, and assures near to physiological fluid-to-tissue ratios.
Journal ArticleDOI
Topological defects in epithelia govern cell death and extrusion.
Thuan Beng Saw,Amin Doostmohammadi,Vincent Nier,Leyla Kocgozlu,Sumesh P. Thampi,Sumesh P. Thampi,Yusuke Toyama,Philippe Marcq,Chwee Teck Lim,Julia M. Yeomans,Benoit Ladoux +10 more
TL;DR: A mechanism for apoptotic cell extrusion is proposed: spontaneously formed topological defects in epithelia govern cell fate, and the ability to control extrusion hotspots by geometrically inducing defects through microcontact printing of patterned monolayers is demonstrated.
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Phototactic guidance of a tissue-engineered soft-robotic ray.
Sung-Jin Park,Mattia Gazzola,Kyung Soo Park,Shirley Park,Valentina Di Santo,Erin L. Blevins,Johan Ulrik Lind,Patrick H. Campbell,Stephanie Dauth,Andrew K. Capulli,Francesco S. Pasqualini,Seungkuk Ahn,Alexander Cho,Hongyan Yuan,Ben M. Maoz,Ragu Vijaykumar,Jeong-Woo Choi,Karl Deisseroth,Karl Deisseroth,George V. Lauder,Lakshminarayanan Mahadevan,Lakshminarayanan Mahadevan,Kevin Kit Parker,Kevin Kit Parker +23 more
TL;DR: In this article, a tissue-engineered ray was constructed from rat cardiomyocytes on an elastomeric body enclosing a microfabricated gold skeleton to mimic the fin deflection patterns of batoid fish.
Journal Article
Phototactic guidance of a tissue-engineered soft-robotic ray.
Sung-Jin Park,Mattia Gazzola,Kyung Soo Park,Shirley Park,Valentina Di Santo,Karl Deisseroth,George V. Lauder,Lakshminarayanan Mahadevan,Kevin Kit Parker +8 more
TL;DR: Inspired by the relatively simple morphological blueprint provided by batoid fish such as stingrays and skates, a biohybrid system that enables an artificial animal—a tissue-engineered ray—to swim and phototactically follow a light cue is created.
References
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TL;DR: Applied extracellular forces stimulate intranuclear movements, resulting in repositioning of nuclear bodies and the associated chromatin within the nucleus, suggesting that the nucleus is a mechanosensitive element within the cell.
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Evaluation of the pressure field on a rigid body entering a quiescent fluid through particle image velocimetry
TL;DR: In this paper, the authors verify the accuracy of indirect pressure measurement from particle image velocimetry in water entry problems by solving the incompressible Navier-Stokes equations.
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Melt behavior on the keyhole front during high speed laser welding
TL;DR: In this article, the flow of molten metal on the front wall of a laser generated welding keyhole has been observed by high speed photography, optically measured by mapping the flow on the liquid surface and theoretically calculated.
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Basics and principles of particle image velocimetry (PIV) for mapping biogenic and biologically relevant flows
TL;DR: The main principles of 2-dimensional digital PIV are clarified in sections on flow types, seeding, illumination, imaging, repetitive correlation analysis, post-processing and result interpretation, with reference to experimental situations.
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Intensity Capping: a simple method to improve cross-correlation PIV results
TL;DR: In this paper, an efficient and easy-to-implement image-enhancement procedure is described to improve particle image velocimetry (PIV) results when bright spots are present.