TALENs: a widely applicable technology for targeted genome editing
J. Keith Joung,Jeffry D. Sander +1 more
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TLDR
The newly-developed transcription activator-like effector nucleases (TALENs) comprise a nonspecific DNA-cleaving nuclease fused to a DNA-binding domain that can be easily engineered so that TALens can target essentially any sequence.Abstract:
Engineered nucleases enable the targeted alteration of nearly any gene in a wide range of cell types and organisms. The newly-developed transcription activator-like effector nucleases (TALENs) comprise a nonspecific DNA-cleaving nuclease fused to a DNA-binding domain that can be easily engineered so that TALENs can target essentially any sequence. The capability to quickly and efficiently alter genes using TALENs promises to have profound impacts on biological research and to yield potential therapeutic strategies for genetic diseases.read more
Citations
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Journal ArticleDOI
Gene editing of stem cells for kidney disease modelling and therapeutic intervention.
TL;DR: The mechanisms of action, in addition to the advantages and disadvantages, of the three major gene editing technologies, namely, CRISPR‐Cas9, zinc finger nucleases and transcription activator‐like effector nucleases are discussed.
Journal ArticleDOI
Abnormalities of hair structure and skin histology derived from CRISPR/Cas9-based knockout of phospholipase C-delta 1 in mice
Yu-min Liu,Wei Liu,Jun-Shuang Jia,Bangzhu Chen,Heng-Wei Chen,Yu Liu,Ya-nan Bie,Peng Gu,Yan Sun,Dong Xiao,Weiwang Gu +10 more
TL;DR: PLCD1 KO mice are produced by CRISPR/Cas9 technology, which provide a new animal model for hair development research, although homozygotes don’t display completely hairless phenotype as expected.
Book ChapterDOI
Integrative Genomic Approaches to Studying Epigenetic Mechanisms of Phenotypic Plasticity in the Aphid
TL;DR: The pea aphid is ideally situated for research into the molecular basis of polyphenism, with a sequenced genome and multiple transcriptomic studies that have begun identifying key molecular regulators of these two polyphenisms.
Posted ContentDOI
Programmable and portable CRISPR-Cas transcriptional activation in bacteria
TL;DR: A novel CRISPR activator, dCas9-AsiA, is identified that could activate gene expression by up to 200-fold across genomic and plasmid targets with diverse promoters after directed evolution and can be ported to other bacteria of clinical and bioindustrial relevance, thus enabling bacterial CRISpra in more application areas.
Book ChapterDOI
The Development and Use of Zinc-Finger Nucleases
TL;DR: Although much of the territory has been ceded in the last few years to the more easily designed TALENs and CRISPR/Cas nucleases, successful ZFNs are still in wide use in a number of applications, including current clinical trials.
References
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Journal ArticleDOI
Breaking the Code of DNA Binding Specificity of TAL-Type III Effectors
Jens Boch,Heidi Scholze,Sebastian Schornack,Angelika Landgraf,Simone Hahn,Sabine Kay,Thomas Lahaye,Anja Nickstadt,Ulla Bonas +8 more
TL;DR: The functionality of a distinct type of DNA binding domain is described and allows the design ofDNA binding domains for biotechnology.
Journal ArticleDOI
Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting
Tomas Cermak,Erin L. Doyle,Michelle Christian,Li-Li Wang,Yong Zhang,Clarice Schmidt,Joshua A. Baller,Nikunj V. Somia,Adam J. Bogdanove,Daniel F. Voytas +9 more
TL;DR: A method and reagents for efficiently assembling TALEN constructs with custom repeat arrays are presented and design guidelines based on naturally occurring TAL effectors and their binding sites are described.
Journal ArticleDOI
A TALE nuclease architecture for efficient genome editing
Jeffrey C. Miller,Siyuan Tan,Guijuan Qiao,Kyle A. Barlow,Jianbin Wang,Danny F Xia,Xiangdong Meng,David Paschon,Elo Leung,Sarah J. Hinkley,Gladys P Dulay,Kevin Hua,Irina Ankoudinova,Gregory J. Cost,Fyodor D. Urnov,H. Steve Zhang,Michael C. Holmes,Lei Zhang,Philip D. Gregory,Edward J. Rebar +19 more
TL;DR: This study identifies TALE truncation variants that efficiently cleave DNA when linked to the catalytic domain of FokI and uses them to generate discrete edits or small deletions within endogenous human NTF3 and CCR5 genes at efficiencies of up to 25%.
Journal ArticleDOI
Genome editing with engineered zinc finger nucleases
TL;DR: A broad range of outcomes has resulted from the application of the same core technology: targeted genome cleavage by engineered, sequence-specific zinc finger nucleases followed by gene modification during subsequent repair.
Journal ArticleDOI
A Simple Cipher Governs DNA Recognition by TAL Effectors
TL;DR: It is shown that a repeat-variable pair of residues specifies the nucleotides in the target site, one pair to one nucleotide, with no apparent context dependence, which represents a previously unknown mechanism for protein-DNA recognition that explains TAL effector specificity, enables target site prediction, and opens prospects for use of TAL effects in research and biotechnology.
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