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Open AccessJournal ArticleDOI

TALENs: a widely applicable technology for targeted genome editing

J. Keith Joung, +1 more
- 01 Jan 2013 - 
- Vol. 14, Iss: 1, pp 49-55
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TLDR
The newly-developed transcription activator-like effector nucleases (TALENs) comprise a nonspecific DNA-cleaving nuclease fused to a DNA-binding domain that can be easily engineered so that TALens can target essentially any sequence.
Abstract: 
Engineered nucleases enable the targeted alteration of nearly any gene in a wide range of cell types and organisms. The newly-developed transcription activator-like effector nucleases (TALENs) comprise a nonspecific DNA-cleaving nuclease fused to a DNA-binding domain that can be easily engineered so that TALENs can target essentially any sequence. The capability to quickly and efficiently alter genes using TALENs promises to have profound impacts on biological research and to yield potential therapeutic strategies for genetic diseases.

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Citations
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Journal ArticleDOI

CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes

Luke A. Gilbert, +12 more
- 18 Jul 2013 - 
TL;DR: The results establish that the CRISPR system can be used as a modular and flexible DNA-binding platform for the recruitment of proteins to a target DNA sequence, revealing the potential of CRISpri as a general tool for the precise regulation of gene expression in eukaryotic cells.
Journal ArticleDOI

CRISPR-Cas systems for editing, regulating and targeting genomes

Jeffry D. Sander, +1 more
- 01 Apr 2014 - 
TL;DR: A modified version of the CRISPR-Cas9 system has been developed to recruit heterologous domains that can regulate endogenous gene expression or label specific genomic loci in living cells, which will undoubtedly transform biological research and spur the development of novel molecular therapeutics for human disease.
Journal ArticleDOI

Efficient genome editing in zebrafish using a CRISPR-Cas system

Woong Y. Hwang, +9 more
- 01 Mar 2013 - 
TL;DR: It is shown that the CRISPR-Cas system functions in vivo to induce targeted genetic modifications in zebrafish embryos with efficiencies similar to those obtained using zinc finger nucleases and transcription activator-like effector nucleases.
Journal ArticleDOI

Efficient multiplex biallelic zebrafish genome editing using a CRISPR nuclease system

Li-En Jao, +2 more
- 20 Aug 2013 - 
TL;DR: An improved CRISPR/Cas system in zebra fish with custom guide RNAs and a zebrafish codon-optimized Cas9 protein that efficiently targeted a reporter transgene Tg(-5.1mnx1:egfp) and four endogenous loci and five genomic loci, resulting in multiple loss-of-function phenotypes in the same injected fish.
Journal ArticleDOI

A guide to genome engineering with programmable nucleases

Hyongbum Kim, +1 more
- 01 May 2014 - 
TL;DR: Known nuclease-specific features are essential for researchers to choose the most appropriate tool for a range of applications, including their composition, targetable sites, specificities and mutation signatures, among other characteristics.
References
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Journal ArticleDOI

The Crystal Structure of TAL Effector PthXo1 Bound to Its DNA Target

Amanda Nga-Sze Mak, +4 more
- 10 Feb 2012 - 
TL;DR: The crystal structure of PthXo1 bound to its DNA target was determined by high-throughput computational structure prediction and validated by heavy-atom derivatization, and illustrates the basis of TAL effector–DNA recognition.
Journal ArticleDOI

Modularly assembled designer TAL effector nucleases for targeted gene knockout and gene replacement in eukaryotes

Ting Li, +7 more
- 01 Aug 2011 - 
TL;DR: These studies expand the realm of verified TALEN activity from cultured human cells to an intact eukaryotic organism and suggest that low-cost, highly dependable dTALENs can assume a significant role for gene modifications of value in human and animal health, agriculture and industry.
Journal ArticleDOI

High-frequency genome editing using ssDNA oligonucleotides with zinc-finger nucleases

Fuqiang Chen, +8 more
- 01 Sep 2011 - 
TL;DR: Methods for using simple ssDNA oligonucleotides in tandem with ZFNs to efficiently produce human cell lines with three distinct genetic outcomes: targeted point mutation, targeted genomic deletion of up to 100 kb and targeted insertion of small genetic elements concomitant with large genomic deletions.
Journal ArticleDOI

Unexpected failure rates for modular assembly of engineered zinc fingers

Cherie L. Ramirez, +12 more
- 01 May 2008 - 
TL;DR: In the version of this correspondence initially published, the two previously published datasets analyzed were labeled with incorrect references in Figure 1b.
Journal ArticleDOI

Enhancing zinc-finger-nuclease activity with improved obligate heterodimeric architectures

Yannick Doyon, +9 more
- 01 Jan 2011 - 
TL;DR: The development and application of a yeast-based selection system designed to functionally interrogate the ZFN dimer interface is reported, identified critical residues involved in dimerization through the isolation of cold-sensitive nuclease domains, and used to engineer ZFNs that have superior cleavage activity while suppressing homodimerization.
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