TALENs: a widely applicable technology for targeted genome editing
J. Keith Joung,Jeffry D. Sander +1 more
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TLDR
The newly-developed transcription activator-like effector nucleases (TALENs) comprise a nonspecific DNA-cleaving nuclease fused to a DNA-binding domain that can be easily engineered so that TALens can target essentially any sequence.Abstract:
Engineered nucleases enable the targeted alteration of nearly any gene in a wide range of cell types and organisms. The newly-developed transcription activator-like effector nucleases (TALENs) comprise a nonspecific DNA-cleaving nuclease fused to a DNA-binding domain that can be easily engineered so that TALENs can target essentially any sequence. The capability to quickly and efficiently alter genes using TALENs promises to have profound impacts on biological research and to yield potential therapeutic strategies for genetic diseases.read more
Citations
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The importance of genomic variation for biodiversity, ecosystems and people
TL;DR: These population and community genomic contexts and their direct and indirect effects on biodiversity, ecosystems and people are explored in the hope of finding solutions for maintaining and improving ecosystem services and nature's contributions to people.
Patent
Rna-guided targeting of genetic and epigenomic regulatory proteins to specific genomic loci
J. Keith Joung,Morgan Maeder +1 more
TL;DR: RNA-guided targeting of heterologous functional domains such as transcriptional activators to specific genomic loci was studied in this article, where the authors proposed RNA-guided RNA-targeting targeting of HOGs.
Journal ArticleDOI
Targeted genome engineering techniques in Drosophila.
Kelly J. Beumer,Dana Carroll +1 more
TL;DR: An overview of the available gene targeting tools and their application in Drosophila is provided, in lieu of simply providing a protocol for gene targeting, to direct the researcher to resources that will allow access to the latest research in this rapidly evolving field.
Book ChapterDOI
Zinc Fingers, TALEs, and CRISPR Systems: A Comparison of Tools for Epigenome Editing.
Charlene Babra Waryah,Colette Moses,Colette Moses,Mahira Arooj,Mahira Arooj,Pilar Blancafort,Pilar Blancafort +6 more
TL;DR: The molecular structure and mechanism of action of ZF, TALE, and CRISPR platforms are summarized and their applications for the locus-specific manipulation of the epigenome are described.
Journal ArticleDOI
Improving the efficiency of precise genome editing with site-specific Cas9-oligonucleotide conjugates.
Xinyu Ling,Bingteng Xie,Bingteng Xie,Xiaoqin Gao,Liying Chang,Wei Zheng,Heqi Chen,Yujia Huang,Linzhi Tan,Mo Li,Mo Li,Tao Liu +11 more
TL;DR: This study has developed methodology to site-specifically conjugate oligonucleotides to recombinant Cas9 protein containing a genetically encoded noncanonical amino acid with orthogonal chemical reactivity, markedly increasing homology-directed repair efficiency in both human cell culture and mouse zygotes.
References
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Journal ArticleDOI
Breaking the Code of DNA Binding Specificity of TAL-Type III Effectors
Jens Boch,Heidi Scholze,Sebastian Schornack,Angelika Landgraf,Simone Hahn,Sabine Kay,Thomas Lahaye,Anja Nickstadt,Ulla Bonas +8 more
TL;DR: The functionality of a distinct type of DNA binding domain is described and allows the design ofDNA binding domains for biotechnology.
Journal ArticleDOI
Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting
Tomas Cermak,Erin L. Doyle,Michelle Christian,Li-Li Wang,Yong Zhang,Clarice Schmidt,Joshua A. Baller,Nikunj V. Somia,Adam J. Bogdanove,Daniel F. Voytas +9 more
TL;DR: A method and reagents for efficiently assembling TALEN constructs with custom repeat arrays are presented and design guidelines based on naturally occurring TAL effectors and their binding sites are described.
Journal ArticleDOI
A TALE nuclease architecture for efficient genome editing
Jeffrey C. Miller,Siyuan Tan,Guijuan Qiao,Kyle A. Barlow,Jianbin Wang,Danny F Xia,Xiangdong Meng,David Paschon,Elo Leung,Sarah J. Hinkley,Gladys P Dulay,Kevin Hua,Irina Ankoudinova,Gregory J. Cost,Fyodor D. Urnov,H. Steve Zhang,Michael C. Holmes,Lei Zhang,Philip D. Gregory,Edward J. Rebar +19 more
TL;DR: This study identifies TALE truncation variants that efficiently cleave DNA when linked to the catalytic domain of FokI and uses them to generate discrete edits or small deletions within endogenous human NTF3 and CCR5 genes at efficiencies of up to 25%.
Journal ArticleDOI
Genome editing with engineered zinc finger nucleases
TL;DR: A broad range of outcomes has resulted from the application of the same core technology: targeted genome cleavage by engineered, sequence-specific zinc finger nucleases followed by gene modification during subsequent repair.
Journal ArticleDOI
A Simple Cipher Governs DNA Recognition by TAL Effectors
TL;DR: It is shown that a repeat-variable pair of residues specifies the nucleotides in the target site, one pair to one nucleotide, with no apparent context dependence, which represents a previously unknown mechanism for protein-DNA recognition that explains TAL effector specificity, enables target site prediction, and opens prospects for use of TAL effects in research and biotechnology.
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