TALENs: a widely applicable technology for targeted genome editing
J. Keith Joung,Jeffry D. Sander +1 more
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TLDR
The newly-developed transcription activator-like effector nucleases (TALENs) comprise a nonspecific DNA-cleaving nuclease fused to a DNA-binding domain that can be easily engineered so that TALens can target essentially any sequence.Abstract:
Engineered nucleases enable the targeted alteration of nearly any gene in a wide range of cell types and organisms. The newly-developed transcription activator-like effector nucleases (TALENs) comprise a nonspecific DNA-cleaving nuclease fused to a DNA-binding domain that can be easily engineered so that TALENs can target essentially any sequence. The capability to quickly and efficiently alter genes using TALENs promises to have profound impacts on biological research and to yield potential therapeutic strategies for genetic diseases.read more
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The essential role of CYP2E1 in metabolism and hepatotoxicity of N,N-dimethylformamide using a novel Cyp2e1 knockout mouse model and a population study.
Zhijun Wu,Qiang Liu,Lei Wang,Min Zheng,Mingyue Guan,Man Zhang,Wenjin Zhao,Chunmin Wang,Songwen Lu,Juan Cheng,Shuguang Leng +10 more
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An Effective Microinjection Method and TALEN-Mediated Genome Editing in Pacific Abalone.
Jianfang Huang,Weiwei You,Zhiwei Xu,Qiuning Yan,Chenggang Shi,Bin Tang,Xuan Luo,Guang Li,Caihuan Ke +8 more
TL;DR: This is the first study to demonstrate site-specific genome editing in abalone, and can serve as a reference for future studies focusing on the functional genomics in mollusks.
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Applying gene-editing technology to elucidate the functional consequence of genetic and epigenetic variation in Alzheimer's disease
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Tomato (Solanum lycopersicum L.) Breeding Strategies for Biotic and Abiotic Stresses
TL;DR: The origin and domestication of tomatoes are described, Biotic resistance and abiotic tolerance strategies are reviewed and the contributing role of biotechnology in generating new tomato cultivars through mutagenesis and the role ofBiotechnology and transgenic approaches are discussed.
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Preclinical short QT syndrome models: studying the phenotype and drug-screening.
Xuehui Fan,Xuehui Fan,Guoqiang Yang,Jacqueline Kowitz,Firat Duru,Ardan M. Saguner,Ibrahim Akin,Xiaobo Zhou,Xiaobo Zhou,Ibrahim El-Battrawy,Ibrahim El-Battrawy +10 more
TL;DR: In this article, the authors reviewed preclinical short QT syndrome (SQTS) models and platforms such as animal models, heterologous expression systems, human-induced pluripotent stem cell-derived cardiomyocyte models and computer models as well as three-dimensional engineered heart tissues.
References
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Journal ArticleDOI
Breaking the Code of DNA Binding Specificity of TAL-Type III Effectors
Jens Boch,Heidi Scholze,Sebastian Schornack,Angelika Landgraf,Simone Hahn,Sabine Kay,Thomas Lahaye,Anja Nickstadt,Ulla Bonas +8 more
TL;DR: The functionality of a distinct type of DNA binding domain is described and allows the design ofDNA binding domains for biotechnology.
Journal ArticleDOI
Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting
Tomas Cermak,Erin L. Doyle,Michelle Christian,Li-Li Wang,Yong Zhang,Clarice Schmidt,Joshua A. Baller,Nikunj V. Somia,Adam J. Bogdanove,Daniel F. Voytas +9 more
TL;DR: A method and reagents for efficiently assembling TALEN constructs with custom repeat arrays are presented and design guidelines based on naturally occurring TAL effectors and their binding sites are described.
Journal ArticleDOI
A TALE nuclease architecture for efficient genome editing
Jeffrey C. Miller,Siyuan Tan,Guijuan Qiao,Kyle A. Barlow,Jianbin Wang,Danny F Xia,Xiangdong Meng,David Paschon,Elo Leung,Sarah J. Hinkley,Gladys P Dulay,Kevin Hua,Irina Ankoudinova,Gregory J. Cost,Fyodor D. Urnov,H. Steve Zhang,Michael C. Holmes,Lei Zhang,Philip D. Gregory,Edward J. Rebar +19 more
TL;DR: This study identifies TALE truncation variants that efficiently cleave DNA when linked to the catalytic domain of FokI and uses them to generate discrete edits or small deletions within endogenous human NTF3 and CCR5 genes at efficiencies of up to 25%.
Journal ArticleDOI
Genome editing with engineered zinc finger nucleases
TL;DR: A broad range of outcomes has resulted from the application of the same core technology: targeted genome cleavage by engineered, sequence-specific zinc finger nucleases followed by gene modification during subsequent repair.
Journal ArticleDOI
A Simple Cipher Governs DNA Recognition by TAL Effectors
TL;DR: It is shown that a repeat-variable pair of residues specifies the nucleotides in the target site, one pair to one nucleotide, with no apparent context dependence, which represents a previously unknown mechanism for protein-DNA recognition that explains TAL effector specificity, enables target site prediction, and opens prospects for use of TAL effects in research and biotechnology.
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