TALENs: a widely applicable technology for targeted genome editing
J. Keith Joung,Jeffry D. Sander +1 more
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TLDR
The newly-developed transcription activator-like effector nucleases (TALENs) comprise a nonspecific DNA-cleaving nuclease fused to a DNA-binding domain that can be easily engineered so that TALens can target essentially any sequence.Abstract:
Engineered nucleases enable the targeted alteration of nearly any gene in a wide range of cell types and organisms. The newly-developed transcription activator-like effector nucleases (TALENs) comprise a nonspecific DNA-cleaving nuclease fused to a DNA-binding domain that can be easily engineered so that TALENs can target essentially any sequence. The capability to quickly and efficiently alter genes using TALENs promises to have profound impacts on biological research and to yield potential therapeutic strategies for genetic diseases.read more
Citations
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Book ChapterDOI
Targeted Editing of Therapeutic Genes Using DNA-Based Transcriptional Activators: Scope and Challenges
TL;DR: The need to gain inspiration from the coordinated chromatin modifications observed in natural cellular environment and design targeting transcriptional activators with epigenetic activity is suggested.
Book ChapterDOI
Functional Genomics Approach Towards Dissecting Out Abiotic Stress Tolerance Trait in Plants
TL;DR: This chapter provides recent findings in the field of functional genomics, thus offering several efficacious methodologies such as next generation sequencing, genome-wide hybridization, gene-inactivation and genome-editing-based strategies in addition to metabolite analysis for discovery as well as validation of the candidate genes.
Journal ArticleDOI
Divalent cations promote TALE DNA-binding specificity
TL;DR: The binding affinity and specificity for a series of TALE proteins under a variety of solution conditions are studied using in vitro fluorescence methods and molecular dynamics simulations to uncover new mechanistic insights into the binding action of TALENs.
Journal ArticleDOI
A Review on Transcriptional Responses of Interactions between Insect Vectors and Plant Viruses
Michael A Catto,Habibu Mugerwa,Brendon K. Myers,Sudeep J. Pandey,Bhabesh Dutta,Rajagopalbabu Srinivasan +5 more
TL;DR: This review compares transcriptional responses in different insect groups following the acquisition of non-persistent, semi- Persistent, and persistent (non-propagative and propagative) plant viruses and identifies parallels and divergences in gene expression patterns.
Journal ArticleDOI
Restoration of dystrophin expression and correction of Duchenne muscular dystrophy by genome editing.
TL;DR: In this paper, the authors outline recent developments in CRISPR/Cas9 genome editing for the correction of Duchenne muscular dystrophy, both in vitro and in vivo, as well as novel delivery methods.
References
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Journal ArticleDOI
Breaking the Code of DNA Binding Specificity of TAL-Type III Effectors
Jens Boch,Heidi Scholze,Sebastian Schornack,Angelika Landgraf,Simone Hahn,Sabine Kay,Thomas Lahaye,Anja Nickstadt,Ulla Bonas +8 more
TL;DR: The functionality of a distinct type of DNA binding domain is described and allows the design ofDNA binding domains for biotechnology.
Journal ArticleDOI
Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting
Tomas Cermak,Erin L. Doyle,Michelle Christian,Li-Li Wang,Yong Zhang,Clarice Schmidt,Joshua A. Baller,Nikunj V. Somia,Adam J. Bogdanove,Daniel F. Voytas +9 more
TL;DR: A method and reagents for efficiently assembling TALEN constructs with custom repeat arrays are presented and design guidelines based on naturally occurring TAL effectors and their binding sites are described.
Journal ArticleDOI
A TALE nuclease architecture for efficient genome editing
Jeffrey C. Miller,Siyuan Tan,Guijuan Qiao,Kyle A. Barlow,Jianbin Wang,Danny F Xia,Xiangdong Meng,David Paschon,Elo Leung,Sarah J. Hinkley,Gladys P Dulay,Kevin Hua,Irina Ankoudinova,Gregory J. Cost,Fyodor D. Urnov,H. Steve Zhang,Michael C. Holmes,Lei Zhang,Philip D. Gregory,Edward J. Rebar +19 more
TL;DR: This study identifies TALE truncation variants that efficiently cleave DNA when linked to the catalytic domain of FokI and uses them to generate discrete edits or small deletions within endogenous human NTF3 and CCR5 genes at efficiencies of up to 25%.
Journal ArticleDOI
Genome editing with engineered zinc finger nucleases
TL;DR: A broad range of outcomes has resulted from the application of the same core technology: targeted genome cleavage by engineered, sequence-specific zinc finger nucleases followed by gene modification during subsequent repair.
Journal ArticleDOI
A Simple Cipher Governs DNA Recognition by TAL Effectors
TL;DR: It is shown that a repeat-variable pair of residues specifies the nucleotides in the target site, one pair to one nucleotide, with no apparent context dependence, which represents a previously unknown mechanism for protein-DNA recognition that explains TAL effector specificity, enables target site prediction, and opens prospects for use of TAL effects in research and biotechnology.
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