TALENs: a widely applicable technology for targeted genome editing
J. Keith Joung,Jeffry D. Sander +1 more
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TLDR
The newly-developed transcription activator-like effector nucleases (TALENs) comprise a nonspecific DNA-cleaving nuclease fused to a DNA-binding domain that can be easily engineered so that TALens can target essentially any sequence.Abstract:
Engineered nucleases enable the targeted alteration of nearly any gene in a wide range of cell types and organisms. The newly-developed transcription activator-like effector nucleases (TALENs) comprise a nonspecific DNA-cleaving nuclease fused to a DNA-binding domain that can be easily engineered so that TALENs can target essentially any sequence. The capability to quickly and efficiently alter genes using TALENs promises to have profound impacts on biological research and to yield potential therapeutic strategies for genetic diseases.read more
Citations
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Type II restriction endonucleases—a historical perspective and more
TL;DR: The mechanisms of sequence recognition and catalysis, and the varied oligomeric modes in which Type II REases act are discussed; the surprising heterogeneity revealed by comparisons of their sequences and structures is described.
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The Genome Landscape of the African Green Monkey Kidney-Derived Vero Cell Line
Naoki Osada,Arihiro Kohara,Toshiyuki Yamaji,Noriko Hirayama,Fumio Kasai,Tsuyoshi Sekizuka,Makoto Kuroda,Kentaro Hanada +7 more
TL;DR: The genome landscape of a Vero cell line was shown, in which 25,877 putative protein-coding genes were identified in the 2.97-Gb genome sequence, and a genomic analysis of Vero cells revealed a female Chlorocebus sabaeus origin and proviral variations of the endogenous simian type D retrovirus.
Journal ArticleDOI
Development of germ-line-specific CRISPR-Cas9 systems to improve the production of heritable gene modifications in Arabidopsis
Yanfei Mao,Zhengjing Zhang,Zhengyan Feng,Pengliang Wei,Hui Zhang,José Ramón Botella,Jian-Kang Zhu,Jian-Kang Zhu +7 more
TL;DR: The design and characterization of a germ-line-specific Cas9 system (GSC) for Arabidopsis gene modification in male gametocytes is reported, constructed using a SPOROCYTELESS genomic expression cassette, and the results suggest that future application of the described GSC system will facilitate the screening for targeted gene modifications, especially lethal mutations in the T2 population.
Journal ArticleDOI
Refining strategies to translate genome editing to the clinic
TL;DR: The advances made in the gene-editing field in recent years are discussed, and priorities that need to be addressed to expand therapeutic genome editing to further disease entities are specified.
Patent
Rna-guided human genome engineering
TL;DR: In this paper, a method of altering a eukaryotic cell is provided including transfecting the eukarotic cell with a nucleic acid encoding RNA complementary to genomic DNA of the EKG, where the cell expresses the RNA and the enzyme, the RNA binds to complementary genomic DNA and the enzymes cleaves the genomic DNA in a site specific manner.
References
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Journal ArticleDOI
Breaking the Code of DNA Binding Specificity of TAL-Type III Effectors
Jens Boch,Heidi Scholze,Sebastian Schornack,Angelika Landgraf,Simone Hahn,Sabine Kay,Thomas Lahaye,Anja Nickstadt,Ulla Bonas +8 more
TL;DR: The functionality of a distinct type of DNA binding domain is described and allows the design ofDNA binding domains for biotechnology.
Journal ArticleDOI
Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting
Tomas Cermak,Erin L. Doyle,Michelle Christian,Li-Li Wang,Yong Zhang,Clarice Schmidt,Joshua A. Baller,Nikunj V. Somia,Adam J. Bogdanove,Daniel F. Voytas +9 more
TL;DR: A method and reagents for efficiently assembling TALEN constructs with custom repeat arrays are presented and design guidelines based on naturally occurring TAL effectors and their binding sites are described.
Journal ArticleDOI
A TALE nuclease architecture for efficient genome editing
Jeffrey C. Miller,Siyuan Tan,Guijuan Qiao,Kyle A. Barlow,Jianbin Wang,Danny F Xia,Xiangdong Meng,David Paschon,Elo Leung,Sarah J. Hinkley,Gladys P Dulay,Kevin Hua,Irina Ankoudinova,Gregory J. Cost,Fyodor D. Urnov,H. Steve Zhang,Michael C. Holmes,Lei Zhang,Philip D. Gregory,Edward J. Rebar +19 more
TL;DR: This study identifies TALE truncation variants that efficiently cleave DNA when linked to the catalytic domain of FokI and uses them to generate discrete edits or small deletions within endogenous human NTF3 and CCR5 genes at efficiencies of up to 25%.
Journal ArticleDOI
Genome editing with engineered zinc finger nucleases
TL;DR: A broad range of outcomes has resulted from the application of the same core technology: targeted genome cleavage by engineered, sequence-specific zinc finger nucleases followed by gene modification during subsequent repair.
Journal ArticleDOI
A Simple Cipher Governs DNA Recognition by TAL Effectors
TL;DR: It is shown that a repeat-variable pair of residues specifies the nucleotides in the target site, one pair to one nucleotide, with no apparent context dependence, which represents a previously unknown mechanism for protein-DNA recognition that explains TAL effector specificity, enables target site prediction, and opens prospects for use of TAL effects in research and biotechnology.
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