The Human Cell Atlas
Aviv Regev,Aviv Regev,Aviv Regev,Sarah A. Teichmann,Sarah A. Teichmann,Sarah A. Teichmann,Eric S. Lander,Eric S. Lander,Eric S. Lander,Ido Amit,Christophe Benoist,Ewan Birney,Bernd Bodenmiller,Bernd Bodenmiller,Peter J. Campbell,Peter J. Campbell,Piero Carninci,Menna R. Clatworthy,Hans Clevers,Bart Deplancke,Ian Dunham,James Eberwine,Roland Eils,Roland Eils,Wolfgang Enard,Andrew Farmer,Lars Fugger,Berthold Göttgens,Nir Hacohen,Nir Hacohen,Muzlifah Haniffa,Martin Hemberg,Seung K. Kim,Paul Klenerman,Paul Klenerman,Arnold R. Kriegstein,Ed S. Lein,Sten Linnarsson,Emma Lundberg,Emma Lundberg,Joakim Lundeberg,Partha P. Majumder,John C. Marioni,John C. Marioni,John C. Marioni,Miriam Merad,Musa M. Mhlanga,Martijn C. Nawijn,Mihai G. Netea,Garry P. Nolan,Dana Pe'er,Anthony Phillipakis,Chris P. Ponting,Stephen R. Quake,Wolf Reik,Wolf Reik,Wolf Reik,Orit Rozenblatt-Rosen,Joshua R. Sanes,Rahul Satija,Ton N. Schumacher,Alex K. Shalek,Alex K. Shalek,Alex K. Shalek,Ehud Shapiro,Padmanee Sharma,Jay W. Shin,Oliver Stegle,Michael R. Stratton,Michael J. T. Stubbington,Fabian J. Theis,Matthias Uhlen,Matthias Uhlen,Alexander van Oudenaarden,Allon Wagner,Fiona M. Watt,Jonathan S. Weissman,Barbara J. Wold,Ramnik J. Xavier,Nir Yosef,Nir Yosef,Human Cell Atlas Meeting Participants +81 more
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TLDR
An open comprehensive reference map of the molecular state of cells in healthy human tissues would propel the systematic study of physiological states, developmental trajectories, regulatory circuitry and interactions of cells, and also provide a framework for understanding cellular dysregulation in human disease.Abstract:
The recent advent of methods for high-throughput single-cell molecular profiling has catalyzed a growing sense in the scientific community that the time is ripe to complete the 150-year-old effort to identify all cell types in the human body. The Human Cell Atlas Project is an international collaborative effort that aims to define all human cell types in terms of distinctive molecular profiles (such as gene expression profiles) and to connect this information with classical cellular descriptions (such as location and morphology). An open comprehensive reference map of the molecular state of cells in healthy human tissues would propel the systematic study of physiological states, developmental trajectories, regulatory circuitry and interactions of cells, and also provide a framework for understanding cellular dysregulation in human disease. Here we describe the idea, its potential utility, early proofs-of-concept, and some design considerations for the Human Cell Atlas, including a commitment to open data, code, and community.read more
Citations
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Informing disease modelling with brain-relevant functional genomic annotations
TL;DR: Reynolds et al. critically assess existing brain-relevant functional genomic annotations and the tools available for integrating such annotations with summary-level genetic association data.
Journal ArticleDOI
CRISPR Tools for Physiology and Cell State Changes: Potential of Transcriptional Engineering and Epigenome Editing.
Christopher T. Breunig,Anna Köferle,Andrea M. Neuner,Maximilian F. Wiesbeck,Valentin Baumann,Stefan H. Stricker +5 more
TL;DR: The molecular basis of DNA targeting tools are discussed and the potential of these new technologies are reviewed before a summary of how these have already been used for the manipulation of cellular states and hypothesize about future applications are summarized.
Journal ArticleDOI
An era of single-cell genomics consortia.
TL;DR: This work introduces existing and nascent single-cell genomics consortia, and presents benefits to necessitate single- cell genomic Consortia in a regional environment to achieve the universal human cell reference dataset.
Journal ArticleDOI
The sound of silence: Transgene silencing in mammalian cell engineering.
Alan Cabera,Hailey I Edelstein,Fokion Glykofrydis,Kasey S. Love,Sebastian Palacios,Joshua C. Tycko,Meng Zhang,Sarah Lensch,Cara Shields,Mark Livingston,Ron Weiss,Huimin Zhao,Karmella A. Haynes,Leonardo Morsut,Yvonne Y. Chen,Ahmad S. Khalil,Wilson W. Wong,James J. Collins,Susan J. Rosser,Karen M. Polizzi,Michael B. Elowitz,Martin Fussenegger,Isaac B. Hilton,Joshua N. Leonard,Lacramioara Bintu,Kate E. Galloway,Tara L. Deans +26 more
TL;DR: In this article , the authors highlight the challenge that transgene silencing poses to the robust engineering of mammalian cells, outline potential molecular mechanisms of silencing, and present approaches for preventing transgogene silencing.
Journal ArticleDOI
New skin for the old RNA-Seq ceremony: the age of single-cell multi-omics.
Maayan Baron,Itai Yanai +1 more
TL;DR: New methods for simultaneously quantifying protein and gene expression at the single-cell level have the power to identify cell types and to classify cell populations.
References
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