Department of Biotechnology

About: Department of Biotechnology is a government organization based out in New Delhi, India. It is known for research contribution in the topics: Population & Silver nanoparticle. The organization has 4800 authors who have published 5033 publications receiving 82022 citations.

Growth Characteristics of Fusarium Spp. Causing Wilt Disease in Psidium Guajava L. in India

Abstract: Fusarium oxysporum f sp psidii and F solani, causal agents of wilt in guava are highly variable pathogens This study was conducted on cultural and physiological (temperature and pH) characters The data revealed that maximum mycelial growth was obtained in potato dextrose agar as semi-solid media ie 7800 mm for F oxysporum f sp psidii; 73 83 mm for F solani, while malt extract broth as liquid broth media ie 1 385 mg mycelia for F oxysporum f sp psidii; 1491 mg for F solani Maximum sporulation was recorded in oatmeal agar and mycological broth The optimum temperature and pH for growth of both Fusarium spp isolates was 28°C and 55 The isolates differed in their colony growth; mycelial mass, macro-conidia, and micro-conidia produced These varia- tions were characters of each of the isolates with respect to cultural and physiological characters

Molecular diversity and functional variability of environmental isolates of Bacillus species

Abstract: In the present study, out of 264 phosphate (P) solubilizing Bacillus strains isolated from apple rhizosphere, only twelve isolates were found to be efficient (showed most of the plant growth promoting activity) which were further characterized at molecular level using 16S rDNA partial gene sequencing. Out of 12 isolates, MZPSB 207 was found to be most efficient P-solubilizing (864.71 μg/ml) isolate which also showed indole acetic acid production (51.83 μg/ml), siderophore production, ammonia production, antagonistic property (against Rhizoctonia solani and Fusarium oxysporum), hydrolytic enzymes productions (protease, chitinase and cellulase), 1-aminocyclopropane-1-carboxylate (ACC) deaminase production (7.7 μm αKB mg-1 h-1). The in-vitro seed germination assay showed that Bacillus (twelve isolates) inoculated seeds showed more seed germination and seedling vigor rate as compared to uninoculated control treatment. For the genetic diversity studies of efficient 12 strains, the polyphasic approach using 16S-rDNA, Repetitive element sequence (rep) based PCR (ERIC-PCR and BOX-PCR) were used. Based on 16S rDNA partial gene sequencing the isolated Bacillus genus was divide into four groups. First group (five isolates), second group (two isolates), third group (three isolates) and fourth group (two isolates) which showed close genetic relatedness to the B. subtilis, B. pumulis, B. megaterium and B. amyloliquefaciens, respectively. The rep PCR fingerprinting showed variability between and within the species. The large variability was showed by ERIC-PCR whereas some variability was showed by BOX-PCR. The results clearly showed that 16S rRNA gene sequencing is unable to discriminate the isolates at strain level. But rep-PCR fingerprinting is excellent tool to characterize and discriminate the strains at the genomic level.