Department of Biotechnology

About: Department of Biotechnology is a government organization based out in New Delhi, India. It is known for research contribution in the topics: Population & Silver nanoparticle. The organization has 4800 authors who have published 5033 publications receiving 82022 citations.

A novel cobiotic-based preventive approach against high-fat diet-induced adiposity, nonalcoholic fatty liver and gut derangement in mice.

Abstract: A novel cobiotic-based preventive approach against high-fat diet-induced adiposity, nonalcoholic fatty liver and gut derangement in mice

Carrier free co-immobilization of glucoamylase and pullulanase as combi-cross linked enzyme aggregates (combi-CLEAs)

Abstract: Herein we report the successful preparation of carrier free co-immobilized form of glucoamylase and pullulanase using combi-CLEAs method from commercial multi-enzyme preparation OPTIMAX® 7525 HP. Glucoamylase and pullulanase were precipitated using ammonium sulfate, followed by cross-linking for 8 h with 2% (v/v) glutaraldehyde to produce insoluble catalytically active co-immobilized form of enzymes. The effects of precipitant type and cross-linking were studied and the biocatalyst was characterized. Scanning electron microscopy analysis showed that co-immobilized form of enzymes was of spherical structure. For starch hydrolytic activity, shift in optimum pH from 5 to 7 and temperature from 60 to 70 °C were observed after co-immobilization of enzymes. After starch hydrolysis reaction in batch mode, 100, 80 and 30% conversions were obtained with co-immobilized enzymes, mixture of separate CLEAs and free enzymes, respectively. Co-immobilization also enhanced the thermal stability and storage stability. After eight reuses with 30 min reaction time, co-immobilized glucoamylase and pullulanase retained 90 and 85% of its initial activity, respectively.

Plant Growth Promoting Rhizobacteria (PGPR): Modern Prospects for Sustainable Agriculture

Abstract: Plant and soil microbiome interactions are in the great demand around the globe. Bacteria that colonize in the plant roots or in the rhizosphere and promote plant growth directly by nutrient immobilization or worked as defense regulator are referred to as plant growth-promoting rhizobacteria (PGPR). During the past couple of decades, PGPR have emerged as a potent alternative to chemical fertilizer in an eco-friendly manner. Therefore, they are abundantly accepted in agriculture, horticulture, silviculture, and environmental cleanup strategies. The rhizosphere ecology is influenced by a myriad of abiotic and biotic factors in natural and agricultural soils, and these factors can, in turn, modulate PGPR effects on plant health. Manipulating this rhizospheric microbiome through rhizo-engineering has materialized as a contemporary methodology to decipher the structural, functional, and ecological behavior of rhizospheric PGPR populations. In this chapter, we have tried to explore the latest developments in the technologies related to PGPR, for its well acceptance for sustainable agriculture and plant health.