Memorial University of Newfoundland

About: Memorial University of Newfoundland is a education organization based out in St. John's, Newfoundland and Labrador, Canada. It is known for research contribution in the topics: Population & Context (language use). The organization has 13818 authors who have published 27785 publications receiving 743594 citations. The organization is also known as: Memorial University & Memorial University of Newfoundland and Labrador.

A Highly Efficient Human Pluripotent Stem Cell Microglia Model Displays a Neuronal-Co-culture-Specific Expression Profile and Inflammatory Response

Abstract: Microglia are increasingly implicated in brain pathology, particularly neurodegenerative disease, with many genes implicated in Alzheimer's, Parkinson's, and motor neuron disease expressed in microglia. There is, therefore, a need for authentic, efficient in vitro models to study human microglial pathological mechanisms. Microglia originate from the yolk sac as MYB-independent macrophages, migrating into the developing brain to complete differentiation. Here, we recapitulate microglial ontogeny by highly efficient differentiation of embryonic MYB-independent iPSC-derived macrophages then co-culture them with iPSC-derived cortical neurons. Co-cultures retain neuronal maturity and functionality for many weeks. Co-culture microglia express key microglia-specific markers and neurodegenerative disease-relevant genes, develop highly dynamic ramifications, and are phagocytic. Upon activation they become more ameboid, releasing multiple microglia-relevant cytokines. Importantly, co-culture microglia downregulate pathogen-response pathways, upregulate homeostatic function pathways, and promote a more anti-inflammatory and pro-remodeling cytokine response than corresponding monocultures, demonstrating that co-cultures are preferable for modeling authentic microglial physiology.

Genetic tagging of humpback whales

Abstract: The ability to recognize individual animals has substantially increased our knowledge of the biology and behaviour of many taxa1. However, not all species lend themselves to this approach, either because of insufficient phenotypic variation or because tag attachment is not feasible. The use of genetic markers (‘tags’) represents a viable alternative to traditional methods of individual recognition, as they are permanent and exist in all individuals. We tested the use of genetic markers as the primary means of identifying individuals in a study of humpback whales in the North Atlantic Ocean. Analysis of six microsatellite loci2,3 among 3,060 skin samples collected throughout this ocean allowed the unequivocal identification of individuals. Analysis of 692 ‘recaptures’, identified by their genotype, revealed individual local and migratory movements of up to 10,000 km, limited exchange among summer feeding grounds, and mixing in winter breeding areas, and also allowed the first estimates of animal abundance based solely on genotypic data. Our study demonstrates that genetic tagging is not only feasible, but generates data (for example, on sex) that can be valuable when interpreting the results of tagging experiments.

Timescale for MeV electron microburst loss during geomagnetic storms

Abstract: [1] Energetic electrons in the outer radiation belt can resonate with intense bursts of whistler-mode chorus emission leading to microburst precipitation into the atmosphere. The timescale for removal of outer zone MeV electrons during the main phase of the October 1998 magnetic storm has been computed by comparing the rate of microburst loss observed on SAMPEX with trapped flux levels observed on Polar. Effective lifetimes are comparable to a day and are relatively independent of L shell. The lifetimes have also been evaluated by theoretical calculations based on quasi-linear scattering by field-aligned waves. Agreement with the observations requires average wide-band wave amplitudes comparable to 100 pT, which is consistent with the intensity of chorus emissions observed under active conditions. MeV electron scattering is most efficient during first-order cyclotron resonance with chorus emissions at geomagnetic latitudes above 30 degrees. Consequently, the zone of MeV microbursts tends to maximize in the prenoon (0400–1200 MLT) sector, since nightside chorus is more strongly confined to the equator.