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TL;DR: Cell viability decreased with an increase in CNT concentration indicating that MWCNTs and its composite can be cytotoxic at higher dosages, and provides further evidence that the bionano interface can be developed for CNT-reinforced HA composites for load-bearing bone implants, drug delivery, and tissue engineering.
Abstract: As a bone mineral component, hydroxyapatite (HA) has been an attractive bioceramic for the reconstruction of hard tissues. However, its poor mechanical properties, including low fracture toughness and tensile strength, have been a substantial challenge to the application of HA for the replacement of load-bearing and/or large bone defects. In this study, HA is reinforced with high-purity and well-functionalized multiwalled carbon nanotubes (MWCNTs; >99 wt%) having an average diameter of 15 nm and length from 10 to 20 μm. The cellular response of these functionalized CNTs and its composites were examined in human osteoblast sarcoma cell lines. Calcium nitrate tetrahydrate (Ca(NO3)2·4H2O) and diammonium hydrogen phosphate ((NH4)2HPO4) were used to synthesize HA in situ. MWCNTs were functionalized by heating at 100°C in 3:1 ratio of sulfuric acid and nitric acid for 60 min with stirring and dispersed in sodium dodecyl benzene sulfonate by sonication. HA particles were produced in MWCNTs solution by adding Ca(NO3)2·4H2O and (NH4)2HPO4 under vigorously stirring conditions. The composite was dried and washed in distilled water followed by heat treatment at 250°C to obtain CNT-HA powder. Physiochemical characterization of the composite material was carried out using Fourier transform infrared spectroscopy, field-emission scanning electron microscopy, energy-dispersive X-ray spectrometer, and X-ray diffractometer. Furthermore, this study investigates the cytotoxic effects of functionalized-MWCNTs (f-MWCNTs) and its composites with HA in human osteoblast sarcoma cell lines. Human osteoblast cells were exposed with different concentrations of f-MWCNTs and its composite with HA. The interactions of f-MWCNT and MWCNT-HA composites were analyzed by 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay. The results indicate no detrimental effect on survival or mitochondrial activity of the osteoblast cells. Cell viability decreased with an increase in CNT concentration indicating that MWCNTs and its composite can be cytotoxic at higher dosages. This result provides further evidence that the bionano interface can be developed for CNT-reinforced HA composites for load-bearing bone implants, drug delivery, and tissue engineering.
34 citations
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TL;DR: In this paper, the thermal analysis of heat-generating battery pack cooled by several coolants is analyzed numerically, including gases, oils, thermal oils, nanofluids, and liquid metals.
Abstract: Thermal analysis of heat-generating battery pack cooled by several coolants is analyzed numerically. The coolant used are gases, oils, thermal oils, nanofluids, and liquid metals to find the best c...
33 citations
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TL;DR: In this paper, a density functional theory (DFT) based analysis of 4-Amino-3-(4-hydroxybenzyl)-1H-1,2,4-triazole-5(4H)-thione (4AHT) has been theoretically analyzed.
33 citations
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TL;DR: In this paper, the effect of different alcohol types on engine performance and emission characteristics was investigated on a single-cylinder, 4-stroke, DI-diesel engine at different speeds at 100 % load.
33 citations
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TL;DR: The preparation of novel surface functionalized halloysite nanotubes (HNTs) with chitosan incorporated Poly (vinyl alcohol) (PVA) nanocomposite films with desirable properties revealed significant enhancement in the mechanical and thermal properties compared with the pristine PVA film.
32 citations
Authors
Showing all 298 results
Name | H-index | Papers | Citations |
---|---|---|---|
Shafiqur Rehman | 46 | 212 | 9437 |
Asif Afzal | 23 | 156 | 1653 |
Balladka Kunhanna Sarojini | 22 | 291 | 2659 |
Mohammad Asif Hussain | 18 | 45 | 1665 |
Sher Afghan Khan | 18 | 248 | 1782 |
M.K. Ramis | 13 | 33 | 443 |
Perveiz Khalid | 13 | 63 | 492 |
M. Anaul Kabir | 12 | 20 | 477 |
Zahid Ansari | 10 | 33 | 404 |
P. R. Thyla | 10 | 44 | 293 |
Mohammad Fazle Azeem | 10 | 44 | 421 |
S. Pradeep | 9 | 19 | 893 |
D. Senthilkumar | 9 | 17 | 336 |
J. Mohan | 9 | 12 | 373 |
A. D. Mohammed Samee | 9 | 12 | 254 |