Saint Louis University

About: Saint Louis University is a education organization based out in St Louis, Missouri, United States. It is known for research contribution in the topics: Population & Health care. The organization has 18927 authors who have published 34895 publications receiving 1267475 citations. The organization is also known as: SLU & St. Louis University.

An early India-Asia contact: Paleomagnetic constraints from Ninetyeast Ridge, ODP Leg 121

Abstract: New paleomagnetic results from sedimentary rock and basement of the Ninetyeast Ridge (Ocean Drilling Program Leg 121, Sites 756-758) detail the northward movement of the Indian plate for the past 80 m.y. Analysis of the combined paleolatitude-age profile indicates a distinct reduction in India9s northward movement rate at 55+ Ma, interpreted as completion of suturing of Greater India and Asia. India9s northward motion slowed from 18-19.5 cm/yr to 4.5 cm/yr for the location of Site 758. Comparison of this profile with paleomagnetic data from the wider Himalayan region indicates that initial contact between northwestern Greater India and southern Asia was already established by Cretaceous-Tertiary time. This supports a possible causal link between the India-Asia convergence and the Deccan Traps extrusion.

Acidic pH enhances the invasive behavior of human melanoma cells.

Abstract: As a consequence of poor perfusion and elevated acid production, the extracellular pH (pHex) of tumors is generally acidic. Despite this, most in vitro experiments are still performed at the relatively alkaline pHex of 7.4. This is significant, because slight changes in pHex can have profound effects on cell phenotype. In this study we examined the effects of mildly acidic conditions on the in vitro invasive potential of two human melanoma cell lines: the highly invasive C8161, and poorly invasive A375P. We observed that culturing of either cell line at acidic pH (6.8) caused dramatic increases in both migration and invasion, as measured with the Membrane Invasion Culture System (MICS). This was not due to a direct effect of pH on the invasive machinery, since cells cultured at normal pH (7.4) and tested at acidic pH did not exhibit increased invasive potential. Similarly, cells cultured at acidic pH were more aggressive than control cells when tested at the same medium pH. These data indicate that culturing of cells at mildly acidic pH induces them to become more invasive. Since acid pH will affect the intracellular pH (pHin) and intracellular calcium ([Ca2+]in), we examined the effect of these parameters on invasion. While changes in [Ca2+]inwere not consistent with invasive potential, the changes in pHin were. While these conditions decrease the overall amount of gelatinases A and B secreted by these cells, there is a consistent and significant increase in the proportion of the activated form of gelatinase B.