A reciprocal repression between ZEB1 and members of the miR-200 family promotes EMT and invasion in cancer cells
Ulrike Burk,Joerg Schubert,Ulrich F. Wellner,Otto Schmalhofer,Elizabeth Vincan,Simone Spaderna,Thomas Brabletz +6 more
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TLDR
Results indicate that ZEB1 triggers an microRNA‐mediated feedforward loop that stabilizes EMT and promotes invasion of cancer cells, and thus explain the strong intratumorous heterogeneity observed in many human cancers.Abstract:
The embryonic programme 'epithelial-mesenchymal transition' (EMT) is thought to promote malignant tumour progression. The transcriptional repressor zinc-finger E-box binding homeobox 1 (ZEB1) is a crucial inducer of EMT in various human tumours, and was recently shown to promote invasion and metastasis of tumour cells. Here, we report that ZEB1 directly suppresses transcription of microRNA-200 family members miR-141 and miR-200c, which strongly activate epithelial differentiation in pancreatic, colorectal and breast cancer cells. Notably, the EMT activators transforming growth factor beta2 and ZEB1 are the predominant targets downregulated by these microRNAs. These results indicate that ZEB1 triggers an microRNA-mediated feedforward loop that stabilizes EMT and promotes invasion of cancer cells. Alternatively, depending on the environmental trigger, this loop might switch and induce epithelial differentiation, and thus explain the strong intratumorous heterogeneity observed in many human cancers.read more
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MiR-124 targets Slug to regulate epithelial-mesenchymal transition and metastasis of breast cancer.
Yong-Jun Liang,Qiu-Yu Wang,Ci-Xiang Zhou,Qian-Qian Yin,Ming He,Xiao-Ting Yu,Dan-Xia Cao,Guo-Qiang Chen,Jianrong He,Qian Zhao +9 more
TL;DR: It is shown that the miR-124 expression is significantly suppressed in human breast cancer specimens, which is reversely correlated to histological grade of the cancer.
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Metastamirs: a stepping stone towards improved cancer management.
TL;DR: The stability of miRNAs in formalin-fixed, paraffin-embedded tissues and body fluids is advantageous for biomarker discovery and validation, and mi RNAs can be extracted from small biopsy specimens, which is a further advantage.
Journal ArticleDOI
Characterization of Epstein-Barr virus miRNAome in nasopharyngeal carcinoma by deep sequencing.
Shu-Jen Chen,Gian-Hung Chen,Yi-Hsuan Chen,Cheng-Yuan Liu,Kai-Ping Chang,Yu-Sun Chang,Hua-Chien Chen +6 more
TL;DR: Analysis of Epstein-Barr virus genomic sequences indicated that the majority of EBV miRNA nucleotide variants resulted from post-transcriptional modifications, suggesting that seed sequence content may be an important factor underlying the differential accumulation of BART miRNAs.
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MicroRNA-138 suppresses epithelial–mesenchymal transition in squamous cell carcinoma cell lines
Xiqiang Liu,Xiqiang Liu,Cheng Wang,Cheng Wang,Zujian Chen,Yi Jin,Yun Wang,Antonia Kolokythas,Yang Dai,Xiaofeng Zhou +9 more
TL;DR: It is demonstrated that down-regulation of miR-138 is a multi-functional molecular regulator and plays major roles in EMT and in HNSCC progression and its effects on cell migration and invasion through targeting RhoC and ROCK2 are observed.
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Epigenetic alterations involved in cancer stem cell reprogramming.
TL;DR: The most relevant epigenetic alterations, from DNA methylation and histone modifications to the recently discovered miRNAs that contribute to the regulation of cancer stem cell features in tumor progression, metastasis and response to chemotherapy are summarized.
References
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Jing Yang,Sendurai A. Mani,Joana Liu Donaher,Sridhar Ramaswamy,Sridhar Ramaswamy,Raphael Itzykson,Christophe Côme,Pierre Savagner,Inna Gitelman,Andrea L. Richardson,Robert A. Weinberg +10 more
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