A simple method for the isolation and purification of total lipides from animal tissues.
TLDR
In this paper, the authors described a simplified version of the method and reported the results of a study of its application to different tissues, including the efficiency of the washing procedure in terms of the removal from tissue lipides of some non-lipide substances of special biochemical interest.About:
This article is published in Journal of Biological Chemistry.The article was published on 1957-05-01 and is currently open access. It has received 59550 citations till now. The article focuses on the topics: Triglyceride transport.read more
Citations
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Muscle and flesh quality traits in wild and farmed Atlantic salmon
Ian A. Johnston,Xuejun Li,Vera L. A. Vieira,David Nickell,Alistair Dingwall,Richard Alderson,Patrick Campbell,Ralph Bickerdike +7 more
TL;DR: It was hypothesised that the higher average values of firmness in the wild than farmed salmon flesh can in part be attributed to higher concentrations of reducible immature collagen crosslinks, which are thought to be enriched with reducible and non-reducible cross-linked collagen.
Journal ArticleDOI
Arf1/COPI machinery acts directly on lipid droplets and enables their connection to the ER for protein targeting
Florian Wilfling,Abdou Rachid Thiam,Abdou Rachid Thiam,Maria-Jesus Olarte,Jing Wang,Rainer D. Beck,Rainer D. Beck,Travis J. Gould,Edward S. Allgeyer,Frederic Pincet,Frederic Pincet,Jörg Bewersdorf,Robert V. Farese,Tobias C. Walther +13 more
TL;DR: A model in which Arf1/COPI machinery acts to control ER-LD connections for localization of key enzymes of TG storage and catabolism is suggested.
Journal ArticleDOI
The role of malic enzyme in the regulation of lipid accumulation in filamentous fungi.
TL;DR: The hypothesis is advanced that NADP(+)-malic enzyme (ME) is an important activity in regulating the extent of lipid accumulation in filamentous fungi, and that the decrease in ME activity occurs due to down-regulation of the ME gene.
Book ChapterDOI
Human apolipoprotein A-I and A-II metabolism
Ernst J. Schaefer,L A Zech,L L Jenkins,T J Bronzert,E A Rubalcaba,Frank T. Lindgren,R L Aamodt,H B Brewer +7 more
TL;DR: The kinetics of the major apolipoproteins of plasma high density lipoproteins, apoA-I and apo-II, were examined in a total of 44 individual tracer studies in 22 normal male and female subjects to show consistency with the following concepts.
Journal ArticleDOI
Effect of Feeding Full Fat Flax and Canola Seeds to Laying Hens on the Fatty Acid Composition of Eggs, Embryos, and Newly Hatched Chicks
G. Cherian,Jeong S. Sim +1 more
TL;DR: The omega-3 fatty acid content of eggs from hens fed flax and canola seed increased significantly and brain tissues of embryos at 15, 17, and 19 days of incubation and on newly hatched chicks were analyzed.
References
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Preparation of lipide extracts from brain tissue.
TL;DR: A simple method is described for the preparation of extracts of total pure lipides from brain tissue by homogenizing the tissue with a chloroform-methanol mixture and washing free of non-lipide contaminants.
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Proteolipides, a new type of tissue lipoproteins; their isolation from brain.
Jordi Folch,Marjorie B. Lees +1 more
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The quantitative estimation of cerebrosides in nervous tissue.
TL;DR: The values for cerebrosides found in the current literature include gangliosides, and other investigators (JOHNSON, MCNABB, and ROSSITER, 1950; CUMINGS, 1953; BLACKWOOD and CUMings, 1954) have neglected the ganglariosides.
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Isolation of brain strandin, a new type of large molecule tissue component.
Jordi Folch,S. Arsove,J.A. Meath +2 more
TL;DR: The isolation from brain tissue of a substance to which the name of strandin has been given for descriptive purposes is reported, which has the property of forming long strands that show perfect orientation under polarized light.
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