A simple method for the isolation and purification of total lipides from animal tissues.
TLDR
In this paper, the authors described a simplified version of the method and reported the results of a study of its application to different tissues, including the efficiency of the washing procedure in terms of the removal from tissue lipides of some non-lipide substances of special biochemical interest.About:
This article is published in Journal of Biological Chemistry.The article was published on 1957-05-01 and is currently open access. It has received 59550 citations till now. The article focuses on the topics: Triglyceride transport.read more
Citations
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A Lipidomic Analysis of Nonalcoholic Fatty Liver Disease
Puneet Puri,Rebecca Baillie,Michelle M. Wiest,Faridoddin Mirshahi,Jayanta Choudhury,Onpan Cheung,Carol Sargeant,Melissa J. Contos,Arun J. Sanyal +8 more
TL;DR: NAFLD is associated with numerous changes in the lipid composition of the liver, and the potential implications are discussed.
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Chromatographic separation, identification, and analysis of phosphatides
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Preparation of rat liver cells. 3. Enzymatic requirements for tissue dispersion.
TL;DR: Isolated perfused rat livers were dispersed by a two-step procedure of Ca2+ removal (probably including the removal of a Ca2-dependent adhesion factor) followed by enzymatic treatment, and cells were active in the synthesis of RNA, protein, glycogen and various metabolites, and showed sensitivity towards steroid and polypeptide hormones.
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PPAR-alpha and PPAR-gamma activators induce cholesterol removal from human macrophage foam cells through stimulation of the ABCA1 pathway.
Giulia Chinetti,Sophie Lestavel,Bocher,Alan T. Remaley,Bernadette Neve,Inés Pineda Torra,Elisabeth Teissier,A Minnich,Michael C. Jaye,Nicolas Duverger,H B Brewer,Jean-Charles Fruchart,Clavey,Bart Staels +13 more
TL;DR: A regulatory role is identified for PPAR-α andPPAR-γ in the first steps of the reverse-cholesterol-transport pathway through the activation of ABCA1-mediated cholesterol efflux in human macrophages.
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Calcium-dependent activation of a multifunctional protein kinase by membrane phospholipids.
TL;DR: The proenzyme of a Ca2+-dependent protease-activated protein kinase previously obtained from mammalian tissues showed an ability to phosphorylate five histone fractions and muscle phosphorylase kinase, and appeared to possess multifunctional catalytic activities.
References
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Preparation of lipide extracts from brain tissue.
TL;DR: A simple method is described for the preparation of extracts of total pure lipides from brain tissue by homogenizing the tissue with a chloroform-methanol mixture and washing free of non-lipide contaminants.
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Proteolipides, a new type of tissue lipoproteins; their isolation from brain.
Jordi Folch,Marjorie B. Lees +1 more
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The quantitative estimation of cerebrosides in nervous tissue.
TL;DR: The values for cerebrosides found in the current literature include gangliosides, and other investigators (JOHNSON, MCNABB, and ROSSITER, 1950; CUMINGS, 1953; BLACKWOOD and CUMings, 1954) have neglected the ganglariosides.
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Isolation of brain strandin, a new type of large molecule tissue component.
Jordi Folch,S. Arsove,J.A. Meath +2 more
TL;DR: The isolation from brain tissue of a substance to which the name of strandin has been given for descriptive purposes is reported, which has the property of forming long strands that show perfect orientation under polarized light.
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