A simple method for the isolation and purification of total lipides from animal tissues.
TLDR
In this paper, the authors described a simplified version of the method and reported the results of a study of its application to different tissues, including the efficiency of the washing procedure in terms of the removal from tissue lipides of some non-lipide substances of special biochemical interest.About:
This article is published in Journal of Biological Chemistry.The article was published on 1957-05-01 and is currently open access. It has received 59550 citations till now. The article focuses on the topics: Triglyceride transport.read more
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Nonalcoholic Steatohepatitis: Association of Insulin Resistance and Mitochondrial Abnormalities
Arun J. Sanyal,Carol Campbell-Sargent,Faridoddin Mirshahi,William B. Rizzo,Melissa J. Contos,Richard K. Sterling,Velimir A. Luketic,Mitchell L. Shiffman,John N. Clore +8 more
TL;DR: Data indicate that peripheral insulin resistance, increased fatty acid beta oxidation, and hepatic oxidative stress are present in both fatty liver and NASH, but NASH alone is associated with mitochondrial structural defects.
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The metabolism of very low density lipoprotein proteins. I. Preliminary in vitro and in vivo observations.
TL;DR: During the first 24 h after injection of 125I-labeled very low density lipoprotein to humans, the decay rate of low densitylipoprotein apoprotein from very lowdensity lipoproteins was faster than that of apolipoprotein glutamic acid, apoipoprotein alanine1 and apolipropoteinAlanine2.
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Ultrasound assisted extraction of food and natural products. Mechanisms, techniques, combinations, protocols and applications. A review.
Farid Chemat,Natacha Rombaut,Anne-Gaëlle Sicaire,Alice Meullemiestre,Anne-Sylvie Fabiano-Tixier,Maryline Abert-Vian +5 more
TL;DR: This review presents a complete picture of current knowledge on ultrasound-assisted extraction in food ingredients and products, nutraceutics, cosmetic, pharmaceutical and bioenergy applications, and applications from laboratory to industry, security, and environmental impacts.
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Lipid extraction by methyl-tert-butyl ether for high-throughput lipidomics
TL;DR: In this paper, the methyl-tert-butyl ether (MTBE) extraction method was used for shotgun profiling of lipid species, and the results showed that the MTBE protocol delivered similar or better recoveries of species of most major lipid classes compared with the "gold-standard" Folch or Bligh and Dyer recipes.
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Mechanism of assembly of the outer membrane of Salmonella typhimurium. Isolation and characterization of cytoplasmic and outer membrane.
TL;DR: Preliminary analysis of the phospholipid composition of the isolated fractions of Salmonella typhimurium showed significant quantitative differences in the relative distribution of the major glycerophosphatides.
References
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Preparation of lipide extracts from brain tissue.
TL;DR: A simple method is described for the preparation of extracts of total pure lipides from brain tissue by homogenizing the tissue with a chloroform-methanol mixture and washing free of non-lipide contaminants.
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Proteolipides, a new type of tissue lipoproteins; their isolation from brain.
Jordi Folch,Marjorie B. Lees +1 more
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The quantitative estimation of cerebrosides in nervous tissue.
TL;DR: The values for cerebrosides found in the current literature include gangliosides, and other investigators (JOHNSON, MCNABB, and ROSSITER, 1950; CUMINGS, 1953; BLACKWOOD and CUMings, 1954) have neglected the ganglariosides.
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Isolation of brain strandin, a new type of large molecule tissue component.
Jordi Folch,S. Arsove,J.A. Meath +2 more
TL;DR: The isolation from brain tissue of a substance to which the name of strandin has been given for descriptive purposes is reported, which has the property of forming long strands that show perfect orientation under polarized light.
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