A simple method for the isolation and purification of total lipides from animal tissues.
TLDR
In this paper, the authors described a simplified version of the method and reported the results of a study of its application to different tissues, including the efficiency of the washing procedure in terms of the removal from tissue lipides of some non-lipide substances of special biochemical interest.About:
This article is published in Journal of Biological Chemistry.The article was published on 1957-05-01 and is currently open access. It has received 59550 citations till now. The article focuses on the topics: Triglyceride transport.read more
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Studying trophic ecology in marine ecosystems using fatty acids: a primer on analysis and interpretation
TL;DR: Over the past three decades, the use of FA develop from a potential tool for delineating food webs to a powerful technique for quantitative assessment of predator diets, allowing both qualitative and quantitative analyses of diet.
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Infant cerebral cortex phospholipid fatty-acid composition and diet.
TL;DR: In this paper, the authors determined the phospholipid fatty acid composition of cerebral cortex grey matter obtained from 20 term and 2 preterm infants who had died of "cot deaths" and related results to the milk diet the infants had received.
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Lipopenia and skin barrier abnormalities in DGAT2-deficient mice.
S. Stone,Heather M. Myers,Steven M. Watkins,Barbara E. Brown,Kenneth R. Feingold,Peter M. Elias,Peter M. Elias,Robert V. Farese +7 more
TL;DR: Dgat2-deficient mice are lipopenic and die soon after birth, apparently from profound reductions in substrates for energy metabolism and from impaired permeability barrier function in the skin, supporting the hypothesis that the two enzymes play fundamentally different roles in mammalian triglyceride metabolism.
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Targeted disruption of hormone-sensitive lipase results in male sterility and adipocyte hypertrophy, but not in obesity
Jun-ichi Osuga,Shun Ishibashi,Teruaki Oka,Hiroaki Yagyu,Ryu-ichi Tozawa,Akihisa Fujimoto,Futoshi Shionoiri,Naoya Yahagi,Frederic B. Kraemer,Osamu Tsutsumi,Nobuhiro Yamada +10 more
TL;DR: It is shown that HSL is required for spermatogenesis but is not the only enzyme that mediates the hydrolysis of triacylglycerol stored in adipocytes, and that adipocytes were significantly enlarged and the size differences between cell and tissue suggests the heterogeneity of adipocytes.
References
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Preparation of lipide extracts from brain tissue.
TL;DR: A simple method is described for the preparation of extracts of total pure lipides from brain tissue by homogenizing the tissue with a chloroform-methanol mixture and washing free of non-lipide contaminants.
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Proteolipides, a new type of tissue lipoproteins; their isolation from brain.
Jordi Folch,Marjorie B. Lees +1 more
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The quantitative estimation of cerebrosides in nervous tissue.
TL;DR: The values for cerebrosides found in the current literature include gangliosides, and other investigators (JOHNSON, MCNABB, and ROSSITER, 1950; CUMINGS, 1953; BLACKWOOD and CUMings, 1954) have neglected the ganglariosides.
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Isolation of brain strandin, a new type of large molecule tissue component.
Jordi Folch,S. Arsove,J.A. Meath +2 more
TL;DR: The isolation from brain tissue of a substance to which the name of strandin has been given for descriptive purposes is reported, which has the property of forming long strands that show perfect orientation under polarized light.
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