A simple method for the isolation and purification of total lipides from animal tissues.
TLDR
In this paper, the authors described a simplified version of the method and reported the results of a study of its application to different tissues, including the efficiency of the washing procedure in terms of the removal from tissue lipides of some non-lipide substances of special biochemical interest.About:
This article is published in Journal of Biological Chemistry.The article was published on 1957-05-01 and is currently open access. It has received 59550 citations till now. The article focuses on the topics: Triglyceride transport.read more
Citations
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Synthetic low and high fat diets for the study of atherosclerosis in the mouse.
TL;DR: The defined high fat diet can be used to study atherosclerosis in the mouse since it produces aortic lesions but reduces or eliminates other pathological changes such as gallstone formation and liver damage.
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Nutritional composition of edible Hawaiian seaweeds
TL;DR: The majority of Hawaiian seaweeds tested containedβ-carotene (vitamin A), and Enteromorpha flexuosa contained the highest amount of vitamin C (3 mg g-1).
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Cisplatin-Induced CD95 Redistribution into Membrane Lipid Rafts of HT29 Human Colon Cancer Cells
Sandrine Lacour,Arlette Hammann,Solène Grazide,Dominique Lagadic-Gossmann,Anne Athias,Odile Sergent,Guy Laurent,Philippe Gambert,Eric Solary,Marie-Thérèse Dimanche-Boitrel +9 more
TL;DR: It is demonstrated that cisplatin activates aSMase and induces ceramide production, which triggers the redistribution of CD95 into the plasma membrane rafts, which contributes to cell death and sensitizes tumor cells to CD95-mediated apoptosis.
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Leptin therapy in insulin-deficient type I diabetes
May-Yun Wang,Lijun Chen,Gregory O. Clark,Young H Lee,Robert Stevens,Olga Ilkayeva,Brett R. Wenner,James R. Bain,Maureen J. Charron,Christopher B. Newgard,Roger H Unger +10 more
TL;DR: It is shown that leptin therapy, like insulin, normalizes the levels of a wide array of hepatic intermediary metabolites in multiple chemical classes, including acylcarnitines, organic acids, amino acids, and acyl CoAs, and it lowers both lipogenic and cholesterologenic transcription factors and enzymes and reduces plasma and tissue lipids.
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Quantitative analysis of sulfolipid (sulfoquinovosyl diglyceride) and galactolipids (monogalactosyl and digalactosyl diglycerides) in plant tissues
P.G. Roughan,Richard D. Batt +1 more
TL;DR: Very small amounts of sulfo- and galacto-lipids can be reproducibly estimated by this method and the sugar component is estimated by the reaction of the lipid on the thin-layer adsorbent with phenol and sulfuric acid.
References
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Preparation of lipide extracts from brain tissue.
TL;DR: A simple method is described for the preparation of extracts of total pure lipides from brain tissue by homogenizing the tissue with a chloroform-methanol mixture and washing free of non-lipide contaminants.
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Proteolipides, a new type of tissue lipoproteins; their isolation from brain.
Jordi Folch,Marjorie B. Lees +1 more
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The quantitative estimation of cerebrosides in nervous tissue.
TL;DR: The values for cerebrosides found in the current literature include gangliosides, and other investigators (JOHNSON, MCNABB, and ROSSITER, 1950; CUMINGS, 1953; BLACKWOOD and CUMings, 1954) have neglected the ganglariosides.
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Isolation of brain strandin, a new type of large molecule tissue component.
Jordi Folch,S. Arsove,J.A. Meath +2 more
TL;DR: The isolation from brain tissue of a substance to which the name of strandin has been given for descriptive purposes is reported, which has the property of forming long strands that show perfect orientation under polarized light.
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