A simple method for the isolation and purification of total lipides from animal tissues.
TLDR
In this paper, the authors described a simplified version of the method and reported the results of a study of its application to different tissues, including the efficiency of the washing procedure in terms of the removal from tissue lipides of some non-lipide substances of special biochemical interest.About:
This article is published in Journal of Biological Chemistry.The article was published on 1957-05-01 and is currently open access. It has received 59550 citations till now. The article focuses on the topics: Triglyceride transport.read more
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Anti-obesity Effects of Three Major Components of Green Tea, Catechins, Caffeine and Theanine, in Mice
TL;DR: Results indicated that at least caffeine and theanine were responsible for the suppressive effect of green tea powder (GTP) on body weight increase and fat accumulation and it was shown that catechins and caffeine were synergistic in anti-obesity activities.
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Sugar transport. II. Characterization of constitutive membrane-bound enzymes II of the Escherichia coli phosphotransferase system.
Werner Kundig,Saul Roseman +1 more
TL;DR: It was shown that II-B, phosphatidylglycerol, and divalent cation interacted to give a sedimentable pellet which catalyzed the phosphorylation of the sugars in the presence of II-A and the phospho-HPr generating system.
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Evidence for an alternative fatty acid desaturation pathway increasing cancer plasticity
Kim Vriens,Stefan Christen,Sweta Parik,Dorien Broekaert,Kazuaki Yoshinaga,Ali Talebi,Jonas Dehairs,Carmen Escalona-Noguero,Roberta Schmieder,Thomas Cornfield,Catriona Charlton,Laura Romero-Pérez,Matteo Rossi,Gianmarco Rinaldi,Martin F. Orth,Ruben Boon,Axelle Kerstens,Suet Ying Kwan,Brandon Faubert,Andrés Méndez-Lucas,Charlotte Kopitz,Ting Chen,Juan Fernández-García,Joao A.G. Duarte,Arndt Schmitz,Patrick Steigemann,Mustapha Najimi,Andrea Hägebarth,Jo A. Van Ginderachter,Etienne Sokal,Naohiro Gotoh,Kwok-Kin Wong,Catherine M. Verfaillie,Rita Derua,Sebastian Munck,Mariia Yuneva,Laura Beretta,Ralph J. DeBerardinis,Johannes V. Swinnen,Leanne Hodson,David Cassiman,Chris Verslype,Sven Christian,Sylvia Grünewald,Thomas G. P. Grunewald,Sarah-Maria Fendt +45 more
TL;DR: In several human and mouse cancer cell lines and carcinomas, a sapienate biosynthesis pathway underpins metabolic plasticity by allowing these cells to bypass stearoyl-CoA desaturase-dependent fatty acid desaturation.
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Interaction of Mixed-Function Oxidation with Biosynthetic Processes
TL;DR: It is assumed that the observed inhibition of gluconeogenesis is not specific for mixed-function oxidation, but may be an example for a more general mechanism of metabolic interdependences involving the extramitochondrial NADPH pool.
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Feeding linseed to increase the n-3 PUFA of pork: fatty acid composition of muscle, adipose tissue, liver and sausages.
TL;DR: It is estimated that the test diet would provide 12 g of long chain n-3 PUFA to the human diet per annum at current pigmeat consumption levels in the UK, about a third of that from oily fish.
References
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Preparation of lipide extracts from brain tissue.
TL;DR: A simple method is described for the preparation of extracts of total pure lipides from brain tissue by homogenizing the tissue with a chloroform-methanol mixture and washing free of non-lipide contaminants.
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Proteolipides, a new type of tissue lipoproteins; their isolation from brain.
Jordi Folch,Marjorie B. Lees +1 more
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The quantitative estimation of cerebrosides in nervous tissue.
TL;DR: The values for cerebrosides found in the current literature include gangliosides, and other investigators (JOHNSON, MCNABB, and ROSSITER, 1950; CUMINGS, 1953; BLACKWOOD and CUMings, 1954) have neglected the ganglariosides.
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Isolation of brain strandin, a new type of large molecule tissue component.
Jordi Folch,S. Arsove,J.A. Meath +2 more
TL;DR: The isolation from brain tissue of a substance to which the name of strandin has been given for descriptive purposes is reported, which has the property of forming long strands that show perfect orientation under polarized light.
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