A simple method for the isolation and purification of total lipides from animal tissues.
TLDR
In this paper, the authors described a simplified version of the method and reported the results of a study of its application to different tissues, including the efficiency of the washing procedure in terms of the removal from tissue lipides of some non-lipide substances of special biochemical interest.About:
This article is published in Journal of Biological Chemistry.The article was published on 1957-05-01 and is currently open access. It has received 59550 citations till now. The article focuses on the topics: Triglyceride transport.read more
Citations
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A linear Lowry--Folin assay for both water-soluble and sodium dodecyl sulfate-solubilized proteins.
TL;DR: Results were obtained with water-insoluble membrane proteins (myelin proteolipid and rhodopsin) solubilized by SDS and Sodium dodecyl sulfate incorporated in the test did not change the albumin standard curve.
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Morphological changes in the adipocyte during fat deposition and mobilization.
TL;DR: It was concluded that the quantity of intracellular fat is an important determinant of the lipid content of a depot; but the volume of an adipocyte is an imperfect measure of its contained lipid.
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A sensitive enzymatic assay for determination of cholesterol in lipid extracts
TL;DR: A procedure for the determination of free and total cholesterol in lipid extracts using cholesterol oxidase and peroxidase to catalyze the reaction of H2O2 with o-dianisidine to yield a colored product.
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Digestibility of starch by rainbow trout: Effects of the physical state of starch and of the intake level
Francoise Bergot,Jeannine Breque +1 more
TL;DR: Starch digestibility in trout was investigated with two diets containing 70% of a common mixture and 30% corn starch which was either native or gelatinized, concluding that starch becomes a valuable source of energy when its digestibility is enhanced by gelatinization.
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Metabolsim of apoB and apoC lipoproteins in man: kinetic studies in normal and hyperlipoproteininemic subjects.
Mones Berman,M Hall,Robert I. Levy,Shlomo Eisenberg,David W. Bilheimer,David W. Bilheimer,Robert D. Phair,Robert D. Phair,R H Goebel,R H Goebel +9 more
TL;DR: The kinetics of apolipoproteins B and C were studied in 14 normal and hyperlipoproteinemic subjects after injection of exogenously-labeled very low density lipoprotein (VLDL) particles, finding a slowdown of the stepwise delipidation process in all hyperlipemic individuals studied.
References
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Preparation of lipide extracts from brain tissue.
TL;DR: A simple method is described for the preparation of extracts of total pure lipides from brain tissue by homogenizing the tissue with a chloroform-methanol mixture and washing free of non-lipide contaminants.
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Proteolipides, a new type of tissue lipoproteins; their isolation from brain.
Jordi Folch,Marjorie B. Lees +1 more
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The quantitative estimation of cerebrosides in nervous tissue.
TL;DR: The values for cerebrosides found in the current literature include gangliosides, and other investigators (JOHNSON, MCNABB, and ROSSITER, 1950; CUMINGS, 1953; BLACKWOOD and CUMings, 1954) have neglected the ganglariosides.
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Isolation of brain strandin, a new type of large molecule tissue component.
Jordi Folch,S. Arsove,J.A. Meath +2 more
TL;DR: The isolation from brain tissue of a substance to which the name of strandin has been given for descriptive purposes is reported, which has the property of forming long strands that show perfect orientation under polarized light.
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