A simple method for the isolation and purification of total lipides from animal tissues.
TLDR
In this paper, the authors described a simplified version of the method and reported the results of a study of its application to different tissues, including the efficiency of the washing procedure in terms of the removal from tissue lipides of some non-lipide substances of special biochemical interest.About:
This article is published in Journal of Biological Chemistry.The article was published on 1957-05-01 and is currently open access. It has received 59550 citations till now. The article focuses on the topics: Triglyceride transport.read more
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Lipid and phospholipid fatty acid composition of plasma, red blood cells, and platelets and how they are affected by dietary lipids: a study of normal subjects from Italy, Finland, and the USA.
TL;DR: It is concluded that the fatty acid compositions of the glycerolphospholipids of plasma, RBCs, and platelets reflect the major dietary fatty acids.
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Lipid analysis by matrix-assisted laser desorption and ionization mass spectrometry: A methodological approach.
TL;DR: The application of MALDI-TOF-MS for the analysis of diacylglycerols, phosphatidylcholines, and (poly)phosphoinositides is reported here for the first time, and it is shown that in a matrix of 2,5-dihydroxybenzoic acid the molecular ions are easily detected even in complex mixtures, and thus, detailed data on the fatty acid composition are provided.
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Prevention of cholesterol gallstone disease by FXR agonists in a mouse model
TL;DR: In this article, the same authors describe the same phenotype in mice lacking the bile acid receptor, FXR, and show that FXR-dependent increases in biliary bile salt and phospholipid concentrations restored cholesterol solubility and thereby prevented gallstone formation.
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Gangliosides in the nervous system.
TL;DR: Gangliosides may regulate cell growth and nerve sprouting, suggesting the potential therapeutic value for some neurological disorders and current ganglioside research is more rapidly growing largely due to advancing methodologies.
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A novel mechanism for the biogenesis of outer membrane vesicles in Gram-negative bacteria
Sandro Roier,Franz G. Zingl,Fatih Cakar,Sanel Durakovic,Paul Kohl,Thomas O. Eichmann,Lisa Klug,Bernhard Gadermaier,Katharina Weinzerl,Ruth Prassl,Achim Lass,Günther Daum,Joachim Reidl,Mario F. Feldman,Stefan Schild +14 more
TL;DR: The results suggest a new general mechanism of OMV biogenesis based on phospholipid accumulation in the outer leaflet of the outer membrane, which is highly conserved among Gram-negative bacteria, provides a means for regulation, can account for OMV formation under all growth conditions, and might have important pathophysiological roles in vivo.
References
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Preparation of lipide extracts from brain tissue.
TL;DR: A simple method is described for the preparation of extracts of total pure lipides from brain tissue by homogenizing the tissue with a chloroform-methanol mixture and washing free of non-lipide contaminants.
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Proteolipides, a new type of tissue lipoproteins; their isolation from brain.
Jordi Folch,Marjorie B. Lees +1 more
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The quantitative estimation of cerebrosides in nervous tissue.
TL;DR: The values for cerebrosides found in the current literature include gangliosides, and other investigators (JOHNSON, MCNABB, and ROSSITER, 1950; CUMINGS, 1953; BLACKWOOD and CUMings, 1954) have neglected the ganglariosides.
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Isolation of brain strandin, a new type of large molecule tissue component.
Jordi Folch,S. Arsove,J.A. Meath +2 more
TL;DR: The isolation from brain tissue of a substance to which the name of strandin has been given for descriptive purposes is reported, which has the property of forming long strands that show perfect orientation under polarized light.
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