A simple method for the isolation and purification of total lipides from animal tissues.
TLDR
In this paper, the authors described a simplified version of the method and reported the results of a study of its application to different tissues, including the efficiency of the washing procedure in terms of the removal from tissue lipides of some non-lipide substances of special biochemical interest.About:
This article is published in Journal of Biological Chemistry.The article was published on 1957-05-01 and is currently open access. It has received 59550 citations till now. The article focuses on the topics: Triglyceride transport.read more
Citations
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IL-7-Induced Glycerol Transport and TAG Synthesis Promotes Memory CD8+ T Cell Longevity
Guoliang Cui,Matthew M. Staron,Simon M. Gray,Ping-Chih Ho,Robert A. Amezquita,Robert A. Amezquita,Jingxia Wu,Susan M. Kaech,Susan M. Kaech +8 more
TL;DR: The metabolic mechanisms by which IL-7 tailors the metabolism of memory T cells to promote their longevity and fast response to rechallenge are uncovered and TAG synthesis is a central component ofIL-7-mediated survival of human and mouse memory CD8+T cells.
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CCR5 Plays a Critical Role in Obesity-Induced Adipose Tissue Inflammation and Insulin Resistance by Regulating Both Macrophage Recruitment and M1/M2 Status
Hironori Kitade,Kazuki Sawamoto,Mayumi Nagashimada,Hiroshi Inoue,Yasuhiko Yamamoto,Yoshimichi Sai,Toshinari Takamura,Hiroshi Yamamoto,Ken-ichi Miyamoto,Henry N. Ginsberg,Naofumi Mukaida,Shuichi Kaneko,Tsuguhito Ota +12 more
TL;DR: It is noteworthy that transplantation of Ccr5−/− bone marrow was sufficient to protect against impaired glucose tolerance and the effects of loss of CCR5 were related to both reduction of total ATM content and an M2-dominant shift in ATM polarization.
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Inter-relationship of lipids transferred by the lipid-transfer protein isolated from human lipoprotein-deficient plasma
TL;DR: Investigation of the protein-mediated transfer of triglyceride, cholesteryl ester, and phosphatidylcholine as a function of the concentration and lipid composition of donor and acceptor lipoproteins concludes that triglyceride and cholesterol ester compete for transfer and that the extent of transfer for each lipid is determined by its relative concentration in the donor particle.
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Tissue triglycerides, insulin resistance, and insulin production: implications for hyperinsulinemia of obesity
TL;DR: In this paper, obesity is associated with both insulin resistance and hyperinsulinemia, which is a condition that compensates for the insulin resistance by maintaining normal glucose homeostasis.
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A review of chromatographic methods for the assessment of phospholipids in biological samples
TL;DR: A summary of the methods that were historically used such as thin layer chromatography, gas chromatography and high-performance liquid chromatography is provided and an introduction to applications of interfacing these traditional chromatographic techniques with mass spectrometry is provided.
References
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Journal ArticleDOI
Preparation of lipide extracts from brain tissue.
TL;DR: A simple method is described for the preparation of extracts of total pure lipides from brain tissue by homogenizing the tissue with a chloroform-methanol mixture and washing free of non-lipide contaminants.
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Proteolipides, a new type of tissue lipoproteins; their isolation from brain.
Jordi Folch,Marjorie B. Lees +1 more
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The quantitative estimation of cerebrosides in nervous tissue.
TL;DR: The values for cerebrosides found in the current literature include gangliosides, and other investigators (JOHNSON, MCNABB, and ROSSITER, 1950; CUMINGS, 1953; BLACKWOOD and CUMings, 1954) have neglected the ganglariosides.
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Isolation of brain strandin, a new type of large molecule tissue component.
Jordi Folch,S. Arsove,J.A. Meath +2 more
TL;DR: The isolation from brain tissue of a substance to which the name of strandin has been given for descriptive purposes is reported, which has the property of forming long strands that show perfect orientation under polarized light.
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