A simple method for the isolation and purification of total lipides from animal tissues.
TLDR
In this paper, the authors described a simplified version of the method and reported the results of a study of its application to different tissues, including the efficiency of the washing procedure in terms of the removal from tissue lipides of some non-lipide substances of special biochemical interest.About:
This article is published in Journal of Biological Chemistry.The article was published on 1957-05-01 and is currently open access. It has received 59550 citations till now. The article focuses on the topics: Triglyceride transport.read more
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An improved mouse model that rapidly develops fibrosis in non‐alcoholic steatohepatitis
Masahiko Matsumoto,Natsuko Hada,Yoshiyuki Sakamaki,Akiko Uno,Toshihiko Shiga,Chiaki Tanaka,Tsuneo Ito,Asao Katsume,Masayuki Sudoh +8 more
TL;DR: An improved CDAHFD model may be a mouse model of rapidly progressive liver fibrosis and be potentially useful for better understanding human NASH disease and in the development of efficient therapies for this condition.
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Rapid, sensitive spectrophotometric method for quantitative determination of sulfatides.
TL;DR: A rapid, sensitive spectrophotometric method for the analysis of sulfatides is described, applicable to theAnalysis of sulfolipids in tissue extracts and gives values similar to those obtained by previously published procedures.
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α-Synuclein occurs in lipid-rich high molecular weight complexes, binds fatty acids, and shows homology to the fatty acid-binding proteins
Ronit Sharon,Matthew S. Goldberg,Ifat Bar-Josef,Rebecca A. Betensky,Jie Shen,Dennis J. Selkoe +5 more
TL;DR: It is concluded that αS has biochemical properties and a structural motif that suggest it is a novel member of the fatty acid-binding protein family and may thus transport fatty acids between the aqueous and membrane phospholipid compartments of the neuronal cytoplasm.
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Binding of arginine-rich (E) apoprotein after recombination with phospholipid vesicles to the low density lipoprotein receptors of fibroblasts.
TL;DR: It was established that the binding activity could be restored to the canine or human E apoproteins following apo-E l DMPC complex formation and phospholipids appear to function not only to bind the apoprotein but also to confer to the E apobrotein the requisite physical state or conformation required for its binding to the cell surface receptor.
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Quaking mouse: isolation and characterization of myelin protein
TL;DR: A new technique, involving final purification on a continuous CsCl gradient, was utilized for the isolation of cerebral myelin from adult quaking mice, littermate controls and young (10‐day‐old) normal mice.
References
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Preparation of lipide extracts from brain tissue.
TL;DR: A simple method is described for the preparation of extracts of total pure lipides from brain tissue by homogenizing the tissue with a chloroform-methanol mixture and washing free of non-lipide contaminants.
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Proteolipides, a new type of tissue lipoproteins; their isolation from brain.
Jordi Folch,Marjorie B. Lees +1 more
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The quantitative estimation of cerebrosides in nervous tissue.
TL;DR: The values for cerebrosides found in the current literature include gangliosides, and other investigators (JOHNSON, MCNABB, and ROSSITER, 1950; CUMINGS, 1953; BLACKWOOD and CUMings, 1954) have neglected the ganglariosides.
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Isolation of brain strandin, a new type of large molecule tissue component.
Jordi Folch,S. Arsove,J.A. Meath +2 more
TL;DR: The isolation from brain tissue of a substance to which the name of strandin has been given for descriptive purposes is reported, which has the property of forming long strands that show perfect orientation under polarized light.
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