A simple method for the isolation and purification of total lipides from animal tissues.
TLDR
In this paper, the authors described a simplified version of the method and reported the results of a study of its application to different tissues, including the efficiency of the washing procedure in terms of the removal from tissue lipides of some non-lipide substances of special biochemical interest.About:
This article is published in Journal of Biological Chemistry.The article was published on 1957-05-01 and is currently open access. It has received 59550 citations till now. The article focuses on the topics: Triglyceride transport.read more
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Physical-chemical behavior of dietary and biliary lipids during intestinal digestion and absorption. 1. Phase behavior and aggregation states of model lipid systems patterned after aqueous duodenal contents of healthy adult human beings
TL;DR: Equilibrium phase diagrams corresponding to aqueous lipid compositions of upper small intestinal contents during lipid digestion and absorption in adult human beings were developed and micellar systems equilibrated rapidly, and their Rh values only slightly influenced by physical-chemical variables of physiological importance.
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Role of platelet activating factor and tumor necrosis factor-alpha in neonatal necrotizing enterocolitis.
Michael S. Caplan,Michael S. Caplan,Xiao-Ming Sun,Xiao-Ming Sun,Wei Hsueh,Wei Hsueh,Joseph R Hageman,Joseph R Hageman +7 more
TL;DR: It is concluded that platelet activating factor and TNF-alpha are elevated in patients with necrotizing enterocolitis and that suppressed platelets activating factor degradation contributes to the increased platelet activates factor levels.
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Pathogenesis of alcohol-induced accumulation of protein in the liver.
TL;DR: Both enhanced protein synthesis and defective export contribute to the ethanol-induced accumulation of liver protein, and the decrease in liver microtubules represents a possible site for impairment of protein export.
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Endothelial cell leukotriene C4 synthesis results from intercellular transfer of leukotriene A4 synthesized by polymorphonuclear leukocytes
TL;DR: The data indicate that endothelial cells synthesize LTC4 from LTA4, and demonstrate a specific PMNL-endothelial cell interaction in which endothelial cell L TC4 synthesis results from the intercellular transfer of L TA4 produced by PMNL.
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Critical Role of stearoyl-CoA desaturase-1 (SCD1) in the Onset of Diet-Induced Hepatic Insulin Resistance
Roger Gutierrez-Juarez,Alessandro Pocai,Claudia Mulas,Hiraku Ono,Sanjay Bhanot,Brett P. Monia,Luciano Rossetti +6 more
TL;DR: Stearoyl-CoA desaturase-1 catalyzes the synthesis of monounsaturated fatty acids from saturated fatty acids and is required for the onset of diet-induced hepatic insulin resistance, and downregulation of Scd1 led to increased Akt phosphorylation and marked decreases in the expression of glucose-6-phosphatase and phosphoenolpyruvate carboxykinase.
References
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Preparation of lipide extracts from brain tissue.
TL;DR: A simple method is described for the preparation of extracts of total pure lipides from brain tissue by homogenizing the tissue with a chloroform-methanol mixture and washing free of non-lipide contaminants.
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Proteolipides, a new type of tissue lipoproteins; their isolation from brain.
Jordi Folch,Marjorie B. Lees +1 more
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The quantitative estimation of cerebrosides in nervous tissue.
TL;DR: The values for cerebrosides found in the current literature include gangliosides, and other investigators (JOHNSON, MCNABB, and ROSSITER, 1950; CUMINGS, 1953; BLACKWOOD and CUMings, 1954) have neglected the ganglariosides.
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Isolation of brain strandin, a new type of large molecule tissue component.
Jordi Folch,S. Arsove,J.A. Meath +2 more
TL;DR: The isolation from brain tissue of a substance to which the name of strandin has been given for descriptive purposes is reported, which has the property of forming long strands that show perfect orientation under polarized light.
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