Comprehensive mapping of long-range interactions reveals folding principles of the human genome.
Erez Lieberman Aiden,Nynke L. van Berkum,Louise Williams,Maxim Imakaev,Tobias Ragoczy,Tobias Ragoczy,Agnes Telling,Agnes Telling,Ido Amit,Bryan R. Lajoie,Peter J. Sabo,Michael O. Dorschner,Richard Sandstrom,Bradley E. Bernstein,Bradley E. Bernstein,Michaël Bender,Mark Groudine,Mark Groudine,Andreas Gnirke,John A. Stamatoyannopoulos,Leonid A. Mirny,Eric S. Lander,Eric S. Lander,Job Dekker +23 more
TLDR
Hi-C is described, a method that probes the three-dimensional architecture of whole genomes by coupling proximity-based ligation with massively parallel sequencing and demonstrates the power of Hi-C to map the dynamic conformations of entire genomes.Abstract:
We describe Hi-C, a method that probes the three-dimensional architecture of whole genomes by coupling proximity-based ligation with massively parallel sequencing. We constructed spatial proximity maps of the human genome with Hi-C at a resolution of 1 megabase. These maps confirm the presence of chromosome territories and the spatial proximity of small, gene-rich chromosomes. We identified an additional level of genome organization that is characterized by the spatial segregation of open and closed chromatin to form two genome-wide compartments. At the megabase scale, the chromatin conformation is consistent with a fractal globule, a knot-free, polymer conformation that enables maximally dense packing while preserving the ability to easily fold and unfold any genomic locus. The fractal globule is distinct from the more commonly used globular equilibrium model. Our results demonstrate the power of Hi-C to map the dynamic conformations of whole genomes.read more
Citations
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Matrix elasticity, cytoskeletal forces and physics of the nucleus: how deeply do cells ‘feel’ outside and in?
TL;DR: Continuity of deformation from the matrix into the cell and further into the cytoskeleton-caged and -linked nucleus also supports the existence of mechanisms that direct processes such as gene expression in the differentiation of stem cells.
Journal ArticleDOI
The Role of Chromosome Domains in Shaping the Functional Genome
Tom Sexton,Giacomo Cavalli +1 more
TL;DR: The current understanding of how these functional genomic "secondary and tertiary structures" form a blueprint for global nuclear architecture and the potential they hold for understanding and manipulating genomic regulation are described.
Journal ArticleDOI
Principles of genome folding into topologically associating domains
TL;DR: The main features of topologically associating domains across species are depicted and the relation between chromatin structure, genome activity, and epigenome is discussed, highlighting mechanistic principles of TAD formation.
Journal ArticleDOI
Allele-defined genome of the autopolyploid sugarcane Saccharum spontaneum L.
Jisen Zhang,Xingtan Zhang,Haibao Tang,Qing Zhang,Xiuting Hua,Xiaokai Ma,Fan Zhu,Tyler Jones,Xin-Guang Zhu,John E. Bowers,Ching Man Wai,Chunfang Zheng,Yan Shi,Shuai Chen,Xiuming Xu,Jingjing Yue,David R. Nelson,Lixian Huang,Zhen Li,Huimin Xu,Dong Zhou,Yongjun Wang,Weichang Hu,Jishan Lin,Youjin Deng,Neha Pandey,Melina Cristina Mancini,Dessireé Zerpa,Julie K. Nguyen,Liming Wang,Liang Yu,Yinghui Xin,Liangfa Ge,Jie Arro,Jennifer Han,Setu Chakrabarty,Marija Pushko,Wenping Zhang,Yanhong Ma,Panpan Ma,Mingju Lv,Faming Chen,Guangyong Zheng,Jingsheng Xu,Zhenhui Yang,Fang Deng,Xuequn Chen,Zhenyang Liao,Xunxiao Zhang,Zhicong Lin,Hai Lin,Hansong Yan,Zheng Kuang,Weimin Zhong,Pingping Liang,Guofeng Wang,Yuan Yuan,Jiaxian Shi,Jinxiang Hou,Jingxian Lin,Jingjing Jin,Peijian Cao,Qiaochu Shen,Qing Jiang,Ping Zhou,Yaying Ma,Xiaodan Zhang,Rongrong Xu,Juan Liu,Yongmei Zhou,Haifeng Jia,Qing Ma,Rui Qi,Zhiliang Zhang,Jingping Fang,Hongkun Fang,Jinjin Song,Mengjuan Wang,Guangrui Dong,Gang Wang,Zheng Chen,Teng Ma,Hong Liu,Singha R. Dhungana,Sarah E. Huss,Xiping Yang,Anupma Sharma,Jhon H. Trujillo,Maria C. Martinez,Matthew E. Hudson,John J. Riascos,Mary A. Schuler,Li Qing Chen,David M. Braun,Lei Li,Qingyi Yu,Jianping Wang,Jianping Wang,Kai Wang,Michael C. Schatz,David Heckerman,Marie-Anne Van Sluys,Glaucia Mendes Souza,Paul H. Moore,David Sankoff,Robert VanBuren,Andrew H. Paterson,Chifumi Nagai,Ray Ming,Ray Ming +109 more
TL;DR: In this article, a haplotype of S. spontaneum, AP85-441, facilitated the assembly of 32 pseudo-chromosomes comprising 8 homologous groups of 4 members each, bearing 35,525 genes with alleles defined.
Journal ArticleDOI
Genome-wide Mapping and Characterization of Notch-Regulated Long Noncoding RNAs in Acute Leukemia
Thomas Trimarchi,Thomas Trimarchi,Erhan Bilal,Panagiotis Ntziachristos,Panagiotis Ntziachristos,Giulia Fabbri,Riccardo Dalla-Favera,Aristotelis Tsirigos,Iannis Aifantis,Iannis Aifantis +9 more
TL;DR: It is shown that one specific Notch-regulated lncRNA, LUNAR1, is required for efficient T-ALL growth in vitro and in vivo due to its ability to enhance IGF1R mRNA expression and sustain IGF1 signaling.
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