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Expansion of SARS-CoV-2-specific Antibody-secreting Cells and Generation of Neutralizing Antibodies in Hospitalized COVID-19 Patients

Renata Varnaitė, +19 more
- 29 May 2020 - 
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TLDR
This study observed a significant expansion of SARS-CoV-2 nucleocapsid protein-specific ASCs in all twenty COVID-19 patients using a multicolor FluoroSpot assay, and found that Sars-Cov-2-specific IgA, IgG and IgM antibody levels positively correlated with SARS -CoV -2-neutralizing antibody titers.
Abstract: 
COVID-19, caused by SARS-CoV-2, emerged in late 2019 and has since become a global pandemic. Pathogen-specific antibodies are typically a major predictor of protective immunity, yet B cell and antibody responses during COVID-19 are not fully understood. Here, we analyzed antibody-secreting cell (ASC) and antibody responses in twenty hospitalized COVID-19 patients. We observed a significant expansion of SARS-CoV-2 nucleocapsid protein-specific ASCs in all twenty COVID-19 patients using a multicolor FluoroSpot assay. Out of the 20 patients, 16 had developed SARS-CoV-2-neutralizing antibodies by the time of sampling. Additionally, we found that SARS-CoV-2-specific IgA, IgG and IgM antibody levels positively correlated with SARS-CoV-2-neutralizing antibody titers. This study constitutes a detailed description of B cell and antibody responses to SARS-CoV-2 in COVID-19, and provides tools to study immune responses to SARS-CoV-2 infection and vaccination.

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Humoral Immune Response to CoronaVac in Turkish Adults

Yasemin Cosgun, +7 more
- 18 Jan 2023 - 
TL;DR: In this article , short-term humoral responses against the SARS-CoV-2 spike (S1) and nucleocapsid (NCP) protein were analyzed in 50 Turkish adults without previous SARS infection after CoronaVac immunization.
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An epidemiological study to determine demographic factors influencing COVID-19 IgG antibody production among the adult population of urban area in Malegaon, Maharashtra - A cross sectional study

Nirmalkumar Adhar Rawandale, +6 more
- 01 Oct 2022 - 
TL;DR: COVID-19 neutralizing antibody prevalence was found to be much higher in the population (96%), which was mostly associated with younger age, gender, diet, and vaccination status of the population.
Posted ContentDOI

Novel universal SARS-CoV DNA vaccine inducing neutralizing antibodies to huCoV-19/WH01, Beta, Delta and Omicron variants and T cells to Bat-CoV

K. Sofia Appelberg, +19 more
TL;DR: A novel universal SARS-CoV-2 DNA vaccine containing the receptor binding domain (RBD) loops from the original hu coV-19/WH01, the Alpha, and the Beta variants, combined with the membrane and nucleoproteins from the hu CoV- 19/WH 01 strain induces a uniquely broad functional immunity.
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Heterologous Booster with BNT162b2 Induced High Specific Antibody Levels in CoronaVac Vaccinees

Letícia Carrijo Masson, +9 more
- 30 Jun 2023 - 
TL;DR: In this article , the authors compared antibody responses induced by ChAdOx1 nCoV-19, CoronaVac, and BNT162b2 vaccines, and concluded that two doses of the studied vaccines induced detectable levels of anti-RBD IgA and IgG and anti-spike IgG in vaccinees.
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Assessing of Humoral Immunity to SARS-COV-2 in Residents of Orenburg During the Epidemic Period

A.S. Pankov, +4 more
TL;DR: The most typical humoral immune response to SARS-CoV-2 has been established, and the age-related characteristics of its formation have been determined and the dependence of the positivity coefficient on the age of the patients was revealed.
References
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Clinical features of patients infected with 2019 novel coronavirus in Wuhan, China

Chaolin Huang, +28 more
- 24 Jan 2020 - 
TL;DR: The epidemiological, clinical, laboratory, and radiological characteristics and treatment and clinical outcomes of patients with laboratory-confirmed 2019-nCoV infection in Wuhan, China, were reported.
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A Novel Coronavirus from Patients with Pneumonia in China, 2019.

Na Zhu, +18 more
- 24 Jan 2020 - 
TL;DR: Human airway epithelial cells were used to isolate a novel coronavirus, named 2019-nCoV, which formed a clade within the subgenus sarbecovirus, Orthocoronavirinae subfamily, which is the seventh member of the family of coronaviruses that infect humans.
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A pneumonia outbreak associated with a new coronavirus of probable bat origin

Peng Zhou, +28 more
- 03 Feb 2020 - 
TL;DR: Identification and characterization of a new coronavirus (2019-nCoV), which caused an epidemic of acute respiratory syndrome in humans in Wuhan, China, and it is shown that this virus belongs to the species of SARSr-CoV, indicates that the virus is related to a bat coronav virus.
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Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR.

Victor M. Corman, +23 more
- 23 Jan 2020 - 
TL;DR: A validated diagnostic workflow for 2019-nCoV is presented, its design relying on close genetic relatedness of 2019- nCoV with SARS coronavirus, making use of synthetic nucleic acid technology.
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Dysregulation of Immune Response in Patients With Coronavirus 2019 (COVID-19) in Wuhan, China.

Chuan Qin, +10 more
- 28 Jul 2020 - 
TL;DR: Investigation of NLR and lymphocyte subsets is helpful in the early screening of critical illness, diagnosis and treatment of COVID-19 and shows the novel coronavirus might mainly act on lymphocytes, especially T lymphocytes.
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Trending Questions (2)
What does it mean to have high levels of Covid antibodies?

SARS-CoV-2-specific IgA, IgG and IgM antibody levels positively correlated with SARS-CoV-2-neutralizing antibody titers, suggesting that SARS-CoV-2-specific antibody levels may reflect the titers of neutralizing antibodies in COVID-19 patients during the acute phase of infection.

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What is Interleukin test Covid?

Lastly, we showed that interleukin 6 (IL-6) and C-reactive protein (CRP) concentrations were higher in serum of patients who were hospitalized for longer, supporting the recent observations that IL-6 and CRP could be used to predict COVID-19 severity.

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