Multivalent nanoparticle networks enable point-of-care detection of human phospholipase-A2 in serum.
Robert Chapman,Yiyang Lin,Mark Burnapp,Andrew Bentham,David Hillier,Abigail Zabron,Shahid A. Khan,Matthew Tyreman,Molly M. Stevens +8 more
TLDR
A rapid and highly sensitive point-of-care lateral flow assay for phospholipase A2 (PLA2) is demonstrated in serum through the enzyme-triggered release of a new class of biotinylated multiarmed polymers from a liposome substrate, enabling the correct diagnosis of pancreatitis in diseased clinical samples against a set of healthy controls using PLA2 activity in a point of care device for the first time.Abstract:
A rapid and highly sensitive point-of-care (PoC) lateral flow assay for phospholipase A2 (PLA2) is demonstrated in serum through the enzyme-triggered release of a new class of biotinylated multiarmed polymers from a liposome substrate. Signal from the enzyme activity is generated by the adhesion of polystreptavidin-coated gold nanoparticle networks to the lateral flow device, which leads to the appearance of a red test line due to the localized surface plasmon resonance effect of the gold. The use of a liposome as the enzyme substrate and multivalent linkers to link the nanoparticles leads to amplification of the signal, as the cleavage of a small amount of lipids is able to release a large amount of polymer linker and adhesion of an even larger amount of gold nanoparticles. By optimizing the molecular weight and multivalency of these biotinylated polymer linkers, the sensitivity of the device can be tuned to enable naked-eye detection of 1 nM human PLA2 in serum within 10 min. This high sensitivity enabl...read more
Citations
More filters
Journal ArticleDOI
Diverse Applications of Nanomedicine
Beatriz Pelaz,Christoph Alexiou,Ramon A. Alvarez-Puebla,Frauke Alves,Frauke Alves,Anne M. Andrews,Sumaira Ashraf,Lajos P. Balogh,Laura Ballerini,Alessandra Bestetti,Cornelia Brendel,Susanna Bosi,Mónica Carril,Warren C. W. Chan,Chunying Chen,Xiaodong Chen,Xiaoyuan Chen,Zhen Cheng,Daxiang Cui,Jianzhong Du,Christian Dullin,Alberto Escudero,Alberto Escudero,Neus Feliu,Mingyuan Gao,Michael D. George,Yury Gogotsi,Arnold Grünweller,Zhongwei Gu,Naomi J. Halas,Norbert Hampp,Roland K. Hartmann,Mark C. Hersam,Patrick Hunziker,Ji Jian,Xingyu Jiang,Philipp Jungebluth,Pranav Kadhiresan,Kazunori Kataoka,Ali Khademhosseini,Jindřich Kopeček,Nicholas A. Kotov,Harald F. Krug,Dong Soo Lee,Claus-Michael Lehr,Kam W. Leong,Xing-Jie Liang,Mei Ling Lim,Luis M. Liz-Marzán,Xiaowei Ma,Paolo Macchiarini,Huan Meng,Helmuth Möhwald,Paul Mulvaney,Andre E. Nel,Shuming Nie,Peter Nordlander,Teruo Okano,Jose Oliveira,Tai Hyun Park,Reginald M. Penner,Maurizio Prato,Maurizio Prato,Víctor F. Puntes,Vincent M. Rotello,Amila Samarakoon,Raymond E. Schaak,Youqing Shen,Sebastian Sjöqvist,Andre G. Skirtach,Andre G. Skirtach,Mahmoud Soliman,Molly M. Stevens,Hsing-Wen Sung,Ben Zhong Tang,Rainer Tietze,Buddhisha Udugama,J. Scott VanEpps,Tanja Weil,Tanja Weil,Paul S. Weiss,Itamar Willner,Yuzhou Wu,Yuzhou Wu,Lily Yang,Zhao Yue,Qian Zhang,Qiang Zhang,Xian-En Zhang,Yuliang Zhao,Xin Zhou,Wolfgang J. Parak +91 more
TL;DR: An overview of recent developments in nanomedicine is provided and the current challenges and upcoming opportunities for the field are highlighted and translation to the clinic is highlighted.
Journal ArticleDOI
Stabilization of Silver and Gold Nanoparticles: Preservation and Improvement of Plasmonic Functionalities.
Hyunho Kang,Joseph T. Buchman,Rebeca S. Rodriguez,Hattie L. Ring,Jiayi He,Kyle C. Bantz,Christy L. Haynes +6 more
TL;DR: This review provides a representative overview of how gold and silver nanoparticles, the most frequently used materials in current plasmonic applications, are stabilized in different application platforms and how the stabilizing agents improve their plAsmonic properties at the same time.
Journal ArticleDOI
A SERS-based lateral flow assay biosensor for highly sensitive detection of HIV-1 DNA
TL;DR: The proposed SERS-based lateral flow assay to quantitatively detect a broad range of genetic diseases with high sensitivity is demonstrated, and human immunodeficiency virus type 1 (HIV-1) DNA was chosen as the specific biomarker.
Journal ArticleDOI
Accelerating the Translation of Nanomaterials in Biomedicine.
Samir Mitragotri,Daniel G. Anderson,Xiaoyuan Chen,Edward Kai-Hua Chow,Dean Ho,Alexander V. Kabanov,Jeffrey M. Karp,Kazunori Kataoka,Chad A. Mirkin,Sarah Hurst Petrosko,Jinjun Shi,Molly M. Stevens,Shouheng Sun,Swee Hin Teoh,Subbu S. Venkatraman,Younan Xia,Shutao Wang,Zhen Gu,Chenjie Xu +18 more
TL;DR: Nano Focus as discussed by the authors discusses the current status and future potential of promising nanomaterials and their translation from the laboratory to the clinic, by highlighting a handful of successful examples.
Journal ArticleDOI
Simultaneous Detection of Dual Nucleic Acids Using a SERS-Based Lateral Flow Assay Biosensor
TL;DR: This is the first report of simultaneous detection of two different DNA mixtures using a SERS-based LFA platform, and this novel detection technique is also a promising multiplex DNA sensing platform for early disease diagnosis.
References
More filters
Journal ArticleDOI
Selective Colorimetric Detection of Polynucleotides Based on the Distance-Dependent Optical Properties of Gold Nanoparticles
TL;DR: A highly selective, colorimetric polynucleotide detection method based on mercaptoalkyloligonucleotide-modified gold nanoparticle probes is reported, which can detect about 10 femtomoles of an oligonucleotide.
Journal ArticleDOI
Calculated Absorption and Scattering Properties of Gold Nanoparticles of Different Size, Shape, and Composition: Applications in Biological Imaging and Biomedicine
TL;DR: While nanorods with a higher aspect ratio along with a smaller effective radius are the best photoabsorbing nanoparticles, the highest scattering contrast for imaging applications is obtained from nanorod of high aspect ratio with a larger effective radius.
Journal ArticleDOI
Gold nanoparticles in chemical and biological sensing.
TL;DR: The advent of AuNP as a sensory element provided a broad spectrum of innovative approaches for the detection of metal ions, small molecules, proteins, nucleic acids, malignant cells, etc. in a rapid and efficient manner.
Journal ArticleDOI
Polyvalent Interactions in Biological Systems: Implications for Design and Use of Multivalent Ligands and Inhibitors.
TL;DR: Polyvalent interactions can be collectively much stronger than corresponding monovalent interactions, and they can provide the basis for mechanisms of both agonizing and antagonizing biological interactions that are fundamentally different from those available inmonovalent systems.