Journal ArticleDOI
Nanopore sensors for nucleic acid analysis
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TLDR
This article reviews the use of nanopore technology in DNA sequencing, genetics and medical diagnostics and suggests that nanopore-based sensors could be competitive with other third-generation DNA sequencing technologies.Abstract:
Nanopore analysis is an emerging technique that involves using a voltage to drive molecules through a nanoscale pore in a membrane between two electrolytes, and monitoring how the ionic current through the nanopore changes as single molecules pass through it. This approach allows charged polymers (including single-stranded DNA, double-stranded DNA and RNA) to be analysed with subnanometre resolution and without the need for labels or amplification. Recent advances suggest that nanopore-based sensors could be competitive with other third-generation DNA sequencing technologies, and may be able to rapidly and reliably sequence the human genome for under $1,000. In this article we review the use of nanopore technology in DNA sequencing, genetics and medical diagnostics.read more
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TL;DR: This review provides a comprehensive survey of recent developments in DNA origami structure, design, assembly, and directed self-assembly, as well as its broad applications.
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Biomimetic Smart Nanopores and Nanochannels
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Journal ArticleDOI
Nanopores: A journey towards DNA sequencing
TL;DR: Past and current studies related to nucleic acid biophysics are surveyed, and will hopefully provoke a discussion of immediate and future prospects for the field.
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Supramolecular self-assemblies as functional nanomaterials
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References
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Journal ArticleDOI
Characterization of nucleic acids by nanopore analysis.
David W. Deamer,Daniel Branton +1 more
TL;DR: Single-stranded DNA and RNA molecules in solution can be driven through a nanoscopic pore by an applied electric field and information about length, composition, structure, and dynamic motion of the molecule can be deduced from modulations of the current blockade.
Journal ArticleDOI
Micropatterning fluid lipid bilayers on solid supports.
TL;DR: Lithographically patterned grids of photoresist, aluminum oxide, or gold on oxidized silicon substrates were used to partition supported lipid bilayers into micrometer-scale arrays of isolated fluid membrane corrals to create arrays of fluid membrane patches with differing compositions.
Journal ArticleDOI
Nanopore DNA sequencing with MspA
Ian M. Derrington,Thomas Z. Butler,Marcus D. Collins,Elizabeth A. Manrao,Mikhail Pavlenok,Michael Niederweis,Jens H. Gundlach +6 more
TL;DR: It is demonstrated that the ionic current through the engineered Mycobacterium smegmatis porin A, MspA, has the ability to distinguish all four DNA nucleotides and resolve single-nucleotides in single- Stranded DNA when double-stranded DNA temporarily holds the nucleotide in the pore constriction.
Journal ArticleDOI
Slowing DNA translocation in a solid-state nanopore
TL;DR: It is demonstrated that DNA translocation speeds can be reduced by an order of magnitude over previous results by controlling the electrolyte temperature, salt concentration, viscosity, and the electrical bias voltage across the nanopore.
Journal ArticleDOI
Controlling protein translocation through nanopores with bio-inspired fluid walls
Erik Yusko,Jay M. Johnson,Sheereen Majd,Panchika Prangkio,Ryan Rollings,Jiali Li,Jerry Yang,Michael Mayer +7 more
TL;DR: It is shown that coating nanopores with fluid bilayer lipids allows the pore diameters to be fine-tuned in sub-nanometre increments, and incorporation of mobile ligands in the lipid conferred specificity and slowed down the translocation of targeted proteins sufficiently to time-resolve translocation events of individual proteins.
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