Journal ArticleDOI
Nanopore sensors for nucleic acid analysis
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TLDR
This article reviews the use of nanopore technology in DNA sequencing, genetics and medical diagnostics and suggests that nanopore-based sensors could be competitive with other third-generation DNA sequencing technologies.Abstract:
Nanopore analysis is an emerging technique that involves using a voltage to drive molecules through a nanoscale pore in a membrane between two electrolytes, and monitoring how the ionic current through the nanopore changes as single molecules pass through it. This approach allows charged polymers (including single-stranded DNA, double-stranded DNA and RNA) to be analysed with subnanometre resolution and without the need for labels or amplification. Recent advances suggest that nanopore-based sensors could be competitive with other third-generation DNA sequencing technologies, and may be able to rapidly and reliably sequence the human genome for under $1,000. In this article we review the use of nanopore technology in DNA sequencing, genetics and medical diagnostics.read more
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Carbon nanomaterials for electronics, optoelectronics, photovoltaics, and sensing
TL;DR: An extensive review of carbon nanomaterials in electronic, optoelectronic, photovoltaic, and sensing devices with a particular focus on the latest examples based on the highest purity samples is presented.
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DNA Origami: Scaffolds for Creating Higher Order Structures
TL;DR: This review provides a comprehensive survey of recent developments in DNA origami structure, design, assembly, and directed self-assembly, as well as its broad applications.
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Biomimetic Smart Nanopores and Nanochannels
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Nanopores: A journey towards DNA sequencing
TL;DR: Past and current studies related to nucleic acid biophysics are surveyed, and will hopefully provoke a discussion of immediate and future prospects for the field.
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Supramolecular self-assemblies as functional nanomaterials
TL;DR: This compilation illustrates how, based on the rules of supramolecular chemistry, the bottom-up approach to design functional objects at the nanoscale is currently producing highly sophisticated materials oriented towards a growing number of applications with high societal impact.
References
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Journal ArticleDOI
Discovering genotypes underlying human phenotypes: past successes for mendelian disease, future approaches for complex disease.
TL;DR: The distribution of types of mutation in mendelian disease genes argues for serious consideration of the early application of a genomic-scale sequence-based approach to association studies and against complete reliance on a positional cloning approach based on a map of anonymous single nucleotide polymorphism haplotypes.
Journal ArticleDOI
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Arnoldus Jan Storm,Jianghua Chen,Xinsheng Sean Ling,Xinsheng Sean Ling,Henny W. Zandbergen,Cees Dekker +5 more
TL;DR: A new technique for fabricating silicon oxide nanopores with single-nanometre precision and direct visual feedback, using state-of-the-art silicon technology and transmission electron microscopy is reported.
Journal ArticleDOI
Human Genome Sequencing Using Unchained Base Reads on Self-Assembling DNA Nanoarrays
Radoje Drmanac,Andrew B. Sparks,Matthew J. Callow,Aaron L. Halpern,Norman L. Burns,Bahram G. Kermani,Paolo Carnevali,Igor Nazarenko,Geoffrey B. Nilsen,George Yeung,Fredrik A. Dahl,Andres Fernandez,Bryan Staker,Krishna Pant,Jonathan Baccash,Adam P. Borcherding,Anushka Brownley,Ryan J. Cedeno,Linsu Chen,Daniel F. Chernikoff,Alex Cheung,Razvan Chirita,Benjamin Curson,Jessica Ebert,Coleen R. Hacker,Robert Hartlage,Brian Hauser,Steve Huang,Yuan Jiang,Vitali Karpinchyk,Mark Koenig,Calvin Kong,Tom Landers,Catherine Le,Jia Liu,Celeste E. McBride,Matt Morenzoni,Robert E. Morey,Karl Mutch,Helena Perazich,Kimberly Perry,Brock A. Peters,Joe Peterson,Charit L. Pethiyagoda,Kaliprasad Pothuraju,Claudia Richter,Abraham M. Rosenbaum,Shaunak Roy,Jay Shafto,Uladzislau Sharanhovich,Karen W. Shannon,Conrad G. Sheppy,Michel Sun,Joseph V. Thakuria,Anne Tran,Dylan Vu,Alexander Wait Zaranek,Xiaodi Wu,Snezana Drmanac,Arnold R. Oliphant,William C. Banyai,Bruce L. Martin,Dennis G. Ballinger,George M. Church,Clifford Reid +64 more
TL;DR: A genome sequencing platform that achieves efficient imaging and low reagent consumption with combinatorial probe anchor ligation chemistry to independently assay each base from patterned nanoarrays of self-assembling DNA nanoballs is described.
Journal ArticleDOI
Graphene as a subnanometre trans-electrode membrane
Slaven Garaj,William A. Hubbard,Alfonso Reina,Jing Kong,Daniel Branton,Jene Andrew Golovchenko +5 more
TL;DR: It is shown that when immersed in an ionic solution, a layer of graphene becomes a new electrochemical structure that is called a trans-electrode, which is an ideal substrate for very high resolution, high throughput nanopore-based single-molecule detectors.
Book ChapterDOI
Real-time DNA sequencing from single polymerase molecules.
Jonas Korlach,Keith Bjornson,Bidhan Chaudhuri,Ronald L. Cicero,Benjamin Flusberg,Jeremy Gray,David P. Holden,Ravi Saxena,Jeffrey Wegener,Stephen Turner +9 more
TL;DR: This chapter will first outline the principle of this single-molecule, real-time (SMRT) DNA sequencing method, followed by descriptions of its underlying components and typical sequencing run conditions.
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