Journal ArticleDOI
Nanopore sensors for nucleic acid analysis
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TLDR
This article reviews the use of nanopore technology in DNA sequencing, genetics and medical diagnostics and suggests that nanopore-based sensors could be competitive with other third-generation DNA sequencing technologies.Abstract:
Nanopore analysis is an emerging technique that involves using a voltage to drive molecules through a nanoscale pore in a membrane between two electrolytes, and monitoring how the ionic current through the nanopore changes as single molecules pass through it. This approach allows charged polymers (including single-stranded DNA, double-stranded DNA and RNA) to be analysed with subnanometre resolution and without the need for labels or amplification. Recent advances suggest that nanopore-based sensors could be competitive with other third-generation DNA sequencing technologies, and may be able to rapidly and reliably sequence the human genome for under $1,000. In this article we review the use of nanopore technology in DNA sequencing, genetics and medical diagnostics.read more
Citations
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Journal ArticleDOI
Theoretical assessment of feasibility to sequence DNA through interlayer electronic tunneling transport at aligned nanopores in bilayer graphene
Jariyanee Prasongkit,Gustavo Troiano Feliciano,Alexandre Reily Rocha,Yuhui He,Tanakorn Osotchan,Rajeev Ahuja,Ralph H. Scheicher +6 more
TL;DR: This work develops a novel approach for sequencing DNA using bilayer graphene to read the interlayer conductance through the layers in the presence of target nucleobases, and assesses the performance of a bilayers graphene nanopore setup for the purpose of DNA sequencing.
Journal ArticleDOI
Automated, Ultra‐Fast Laser‐Drilling of Nanometer Scale Pores and Nanopore Arrays in Aqueous Solutions
TL;DR: In this paper, the principal mechanism governing material etching and pore formation using light is investigated and it is found that the process is extremely sensitive to the relative content of Si over N atoms in the amorphous membrane, produced by chemical vapor deposition.
Journal ArticleDOI
DNA sequencing by nanopores: advances and challenges
TL;DR: In this review, recent progress in the nanopore sequencing field is discussed with a focus on the nature of nanopores and on sensing mechanisms during the translocation.
Journal ArticleDOI
Surface modification of graphene nanopores for protein translocation.
Yuping Shan,Purushottam B. Tiwari,Padmini Krishnakumar,Ivan Vlassiouk,Wei Li,Xuewen Wang,Yesim Darici,Stuart Lindsay,Hongda Wang,Sergei N. Smirnov,Jin He +10 more
TL;DR: The ion current signature of translocation events was complex, suggesting that a series of interactions between the protein and pores occurs during the process, and is consistent with a small surface charge induced by the formation of carboxyl groups.
Journal ArticleDOI
Electron beam-assisted healing of nanopores in magnesium alloys
TL;DR: It is demonstrated that nanopores can be successfully fabricated in Mg alloys via focused electron beam (e-beam) technology via unambiguous evidence that layer-by-layer growth of atomic planes at the nanopore periphery occurs when the e-beam is spread out, leading to the shrinkage and eventual disappearance of nanopores.
References
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TL;DR: A public database of common variation in the human genome: more than one million single nucleotide polymorphisms for which accurate and complete genotypes have been obtained in 269 DNA samples from four populations, including ten 500-kilobase regions in which essentially all information about common DNA variation has been extracted.
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