Journal ArticleDOI
Nanopore sensors for nucleic acid analysis
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TLDR
This article reviews the use of nanopore technology in DNA sequencing, genetics and medical diagnostics and suggests that nanopore-based sensors could be competitive with other third-generation DNA sequencing technologies.Abstract:
Nanopore analysis is an emerging technique that involves using a voltage to drive molecules through a nanoscale pore in a membrane between two electrolytes, and monitoring how the ionic current through the nanopore changes as single molecules pass through it. This approach allows charged polymers (including single-stranded DNA, double-stranded DNA and RNA) to be analysed with subnanometre resolution and without the need for labels or amplification. Recent advances suggest that nanopore-based sensors could be competitive with other third-generation DNA sequencing technologies, and may be able to rapidly and reliably sequence the human genome for under $1,000. In this article we review the use of nanopore technology in DNA sequencing, genetics and medical diagnostics.read more
Citations
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Journal ArticleDOI
Mechanical exfoliation of track-etched two-dimensional layered materials for the fabrication of ultrathin nanopores
TL;DR: Mechanical exfoliation of ion-track-etched two-dimensional layered materials yields nanometer-thin nanoporous sheets that can be suspended atop a silicon window to controllably fabricate single- or multi-pore nanofluidic devices.
Journal ArticleDOI
γ-Hemolysin Nanopore Is Sensitive to Guanine-to-Inosine Substitutions in Double-Stranded DNA at the Single-Molecule Level
TL;DR: The γ-HL protein nanopore is able to translocate dsDNA at about ∼20 bp/ms, and the unique current-signature of captured ds DNA can directly distinguish guanine-to-inosine substitutions at the single-molecule level with ∼99% accuracy.
Journal ArticleDOI
A long DNA segment in a linear nanoscale Paul trap.
TL;DR: It is shown that a stretching field in the axial direction can effectively prevent the rotations and increase the confinement stability of the line charge in the Paul trap.
Journal ArticleDOI
Two-step breakdown of a SiN membrane for nanopore fabrication: Formation of thin portion and penetration.
TL;DR: In this paper, a two-step breakdown (TSB) method was proposed for the stable fabrication of a single large nanopore due to the risk of generating multiple nanopores.
Journal ArticleDOI
Challenges and Opportunities for Clustered Regularly Interspaced Short Palindromic Repeats Based Molecular Biosensing.
Mengdi Bao,Qun Chen,Zhiheng Xu,Erik C. Jensen,Changyue Liu,Jacob T Waitkus,Xi Yuan,Qian He,Peiwu Qin,Ke Du +9 more
TL;DR: In this article, the authors review the history of CRISPR molecular biosensors, followed by the current status of nucleic acid-based detection methods, and discuss the current challenges pertaining to CRISpl-based nucleic acids detection, and then discuss the recent breakthroughs addressing these challenges.
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TL;DR: A public database of common variation in the human genome: more than one million single nucleotide polymorphisms for which accurate and complete genotypes have been obtained in 269 DNA samples from four populations, including ten 500-kilobase regions in which essentially all information about common DNA variation has been extracted.
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