Journal ArticleDOI
Nanopore sensors for nucleic acid analysis
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TLDR
This article reviews the use of nanopore technology in DNA sequencing, genetics and medical diagnostics and suggests that nanopore-based sensors could be competitive with other third-generation DNA sequencing technologies.Abstract:
Nanopore analysis is an emerging technique that involves using a voltage to drive molecules through a nanoscale pore in a membrane between two electrolytes, and monitoring how the ionic current through the nanopore changes as single molecules pass through it. This approach allows charged polymers (including single-stranded DNA, double-stranded DNA and RNA) to be analysed with subnanometre resolution and without the need for labels or amplification. Recent advances suggest that nanopore-based sensors could be competitive with other third-generation DNA sequencing technologies, and may be able to rapidly and reliably sequence the human genome for under $1,000. In this article we review the use of nanopore technology in DNA sequencing, genetics and medical diagnostics.read more
Citations
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Journal ArticleDOI
Electro-osmotic flow through nanopores in thin and ultrathin membranes.
TL;DR: Theoretical study of how the electro-osmotic fluid velocity in a charged cylindrical nanopore in a thin solid state membrane depends on the pore's geometry, membrane charge, and electrolyte concentration finds that when the pores' length is comparable to its diameter, the velocity profile develops a concave shape.
Journal ArticleDOI
Toward the Responsible Development and Commercialization of Sensor Nanotechnologies
Tarek R. Fadel,Dorothy Farrell,Lisa E. Friedersdorf,Mark H. Griep,Mark D. Hoover,Michael A. Meador,Meyya Meyyappan +6 more
TL;DR: Recent efforts by the Sensors NSI aimed at promoting the successful development and commercialization of nanosensors will be reviewed and examples of sensor nanotechnologies will be highlighted.
Journal ArticleDOI
Channel Size Conversion of Phi29 DNA-Packaging Nanomotor for Discrimination of Single- and Double-Stranded Nucleic Acids
TL;DR: This finding of size alterations in reengineered motor channels expands the potential application of the phi29 DNA-packaging motor in nanomedicine, nanobiotechnology, and high-throughput single-pore DNA sequencing.
Patent
Hairpin loop method for double strand polynucleotide sequencing using transmembrane pores
TL;DR: In this article, a bridging moiety was used to link two strands of the double-stranded target polynucleotide with a polynotide binding protein and then the target was sequenced using a transmembrane pore.
Journal ArticleDOI
Electrochemical Resistive-Pulse Sensing.
Rongrong Pan,Rongrong Pan,Keke Hu,Keke Hu,Dechen Jiang,Uri Samuni,Uri Samuni,Michael V. Mirkin,Michael V. Mirkin +8 more
TL;DR: A new version of the resistive-pulse technique based on the use of carbon nano-pipettes (CNP) that enables qualitative and quantitative analysis of the electroactive material they contain and indicates the possibility of single-entity measurements in biological systems.
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TL;DR: A public database of common variation in the human genome: more than one million single nucleotide polymorphisms for which accurate and complete genotypes have been obtained in 269 DNA samples from four populations, including ten 500-kilobase regions in which essentially all information about common DNA variation has been extracted.
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