Journal ArticleDOI
Nanopore sensors for nucleic acid analysis
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TLDR
This article reviews the use of nanopore technology in DNA sequencing, genetics and medical diagnostics and suggests that nanopore-based sensors could be competitive with other third-generation DNA sequencing technologies.Abstract:
Nanopore analysis is an emerging technique that involves using a voltage to drive molecules through a nanoscale pore in a membrane between two electrolytes, and monitoring how the ionic current through the nanopore changes as single molecules pass through it. This approach allows charged polymers (including single-stranded DNA, double-stranded DNA and RNA) to be analysed with subnanometre resolution and without the need for labels or amplification. Recent advances suggest that nanopore-based sensors could be competitive with other third-generation DNA sequencing technologies, and may be able to rapidly and reliably sequence the human genome for under $1,000. In this article we review the use of nanopore technology in DNA sequencing, genetics and medical diagnostics.read more
Citations
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Patent
Compositions and methods for polynucleotide sequencing
Eric Stava,Jens H. Gundlach,Mandell Jeffrey G,Gunderson Kevin L,Ian M. Derrington,Hosein Mohimani +5 more
TL;DR: In this article, the sequence of the target polynucleotide was characterised using the fractional translocation steps of the translocation through a pore, and the pore was used to characterize the sequence.
Patent
Systems and devices for molecule sensing and method of manufacturing thereof
TL;DR: In this paper, the authors describe a device for molecule sensing that includes a first electrode separated from a second electrode by a dielectric layer, where at least one opening (e.g., trench) is created or otherwise created into the layer exposing a tunnel junction there between whereby target molecules in solution can bind across the tunnel junction.
Journal ArticleDOI
From Water Solutions to Ionic Liquids with Solid State Nanopores as a Perspective to Study Transport and Translocation Phenomena
Sanjin Marion,Nataša Vučemilović-Alagić,Mario Špadina,Aleksandra Radenovic,Ana-Sunčana Smith +4 more
TL;DR: In this article, it is argued that ionic liquid and nanopores can be combined to provide new nanofluidic functionalities, as well as to help resolve some of the pertinent problems in understanding transport phenomena in confined ionic liquids and providing better control of the speed of translocating analytes.
Journal ArticleDOI
Electrohydrodynamic Flow through a 1 mm2 Cross-Section Pore Placed in an Ion-Exchange Membrane
TL;DR: The development of a theoretical model of liquid flows resulting from ion interactions while focusing on the so-called electrohydrodynamic (EHD) flow is reported on and the transient phenomena associated with ionic responses are theoretically elucidated.
Journal ArticleDOI
Fluctuating bottleneck model studies on kinetics of DNA escape from α-hemolysin nanopores.
TL;DR: The proposed fluctuation bottleneck model has caught some key features regarding the long time kinetics of DNA escape through a nanopore and it might provide a sound starting point to study much wider problems involving anomalous dynamics in confined fluctuating channels.
References
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TL;DR: A public database of common variation in the human genome: more than one million single nucleotide polymorphisms for which accurate and complete genotypes have been obtained in 269 DNA samples from four populations, including ten 500-kilobase regions in which essentially all information about common DNA variation has been extracted.
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