Journal ArticleDOI
Nanopore sensors for nucleic acid analysis
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TLDR
This article reviews the use of nanopore technology in DNA sequencing, genetics and medical diagnostics and suggests that nanopore-based sensors could be competitive with other third-generation DNA sequencing technologies.Abstract:
Nanopore analysis is an emerging technique that involves using a voltage to drive molecules through a nanoscale pore in a membrane between two electrolytes, and monitoring how the ionic current through the nanopore changes as single molecules pass through it. This approach allows charged polymers (including single-stranded DNA, double-stranded DNA and RNA) to be analysed with subnanometre resolution and without the need for labels or amplification. Recent advances suggest that nanopore-based sensors could be competitive with other third-generation DNA sequencing technologies, and may be able to rapidly and reliably sequence the human genome for under $1,000. In this article we review the use of nanopore technology in DNA sequencing, genetics and medical diagnostics.read more
Citations
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Journal ArticleDOI
Empowering single-molecule analysis with self-assembled DNA nanostructures
TL;DR: In this paper, a review of advances in using self-assembled DNA nanostructures for single-molecule analysis is presented, which can not only act as a part of a bottom-up constructed analytic system, but also serve as a defined nanoobject for the calibration of new methodologies.
Journal ArticleDOI
A potential barrier in the diffusion of nanoparticles in ordered polymer networks.
Yu Lu,Guo-Hui Hu +1 more
TL;DR: In this paper, the authors employed the single-particle Dissipative Particle Dynamics (DPD) to study the diffusion of nanoparticles in an unentangled ordered polymer network.
Journal ArticleDOI
Exponential Increase in an Ionic Signal: A Dominant Role of the Space Charge Effect on the Outer Surface of Nanochannels.
TL;DR: Using electroneutral peptide nucleic acid (PNA) and negative-charged DNA, this paper verified a dominant space charge effect on an ionic signal on the outer surface of nanochannels.
Proceedings ArticleDOI
NanoBLASTer: Fast alignment and characterization of Oxford Nanopore single molecule sequencing reads
TL;DR: The quality of the Oxford Nanopores long DNA sequence reads has been, to date, lower than other technologies, causing great interest to develop new algorithms that can make use of the data, and a new read aligner specifically designed for long nanopore reads is developed.
Journal ArticleDOI
Dynamic single-molecule sensing by actively tuning binding kinetics for ultrasensitive biomarker detection
TL;DR: This work establishes a rapid and ultrasensitive approach by actively tuning binding kinetics and accelerating the mass transportation of analyte molecules onto the sensor surfaces via nanoparticle micromanipulations, which permits extremely sensitive measurements within clinically acceptable assay time.
References
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TL;DR: A public database of common variation in the human genome: more than one million single nucleotide polymorphisms for which accurate and complete genotypes have been obtained in 269 DNA samples from four populations, including ten 500-kilobase regions in which essentially all information about common DNA variation has been extracted.
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