Journal ArticleDOI
Nanopore sensors for nucleic acid analysis
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TLDR
This article reviews the use of nanopore technology in DNA sequencing, genetics and medical diagnostics and suggests that nanopore-based sensors could be competitive with other third-generation DNA sequencing technologies.Abstract:
Nanopore analysis is an emerging technique that involves using a voltage to drive molecules through a nanoscale pore in a membrane between two electrolytes, and monitoring how the ionic current through the nanopore changes as single molecules pass through it. This approach allows charged polymers (including single-stranded DNA, double-stranded DNA and RNA) to be analysed with subnanometre resolution and without the need for labels or amplification. Recent advances suggest that nanopore-based sensors could be competitive with other third-generation DNA sequencing technologies, and may be able to rapidly and reliably sequence the human genome for under $1,000. In this article we review the use of nanopore technology in DNA sequencing, genetics and medical diagnostics.read more
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Journal ArticleDOI
Ion Transport in Confined Geometries below the Nanoscale: Access Resistance Dominates Protein Channel Conductance in Diluted Solutions
TL;DR: It is shown that lipid surface charges modify the ion distribution and determine the value of AR, indicating that lipid molecules are more than passive scaffolds even in the case of large transmembrane proteins.
Journal ArticleDOI
Correlation dynamics and enhanced signals for the identification of serial biomolecules and DNA bases.
Towfiq Ahmed,Jason T. Haraldsen,Jason T. Haraldsen,John J. Rehr,Massimiliano Di Ventra,Ivan K. Schuller,Alexander V. Balatsky +6 more
TL;DR: This work proposes a specific multilayered graphene-based nanopore device architecture for the recognition of single biomolecules and implements a new 'multi-point cross-correlation' technique for identification of DNA bases or other molecules on the single molecular level.
Journal ArticleDOI
Fingerprinting of Peptides with a Large Channel of Bacteriophage Phi29 DNA Packaging Motor
TL;DR: The utility of phi29 motor channel for fingerprinting of various peptides using single molecule electrophysiological assays is reported and the translocation of peptides is proved unequivocally by single molecule fluorescence imaging.
Journal ArticleDOI
Stable fabrication of a large nanopore by controlled dielectric breakdown in a high-pH solution for the detection of various-sized molecules.
TL;DR: In this article, a simple and inexpensive nanopore fabrication method utilizing dielectric breakdown of a SiN membrane under high-pH conditions (pH ≥ 11.3) was used to overcome two serious problems: the generation of multiple nanopores and the non-opening failure of a nanopore.
Journal ArticleDOI
Noncovalent Approach toward the Construction of Nanofluidic Diodes with pH-Reversible Rectifying Properties: Insights from Theory and Experiment
Gonzalo Pérez-Mitta,Alberto G. Albesa,Facundo Matias Gilles,Maria Eugenia Toimil-Molares,Christina Trautmann,Christina Trautmann,Omar Azzaroni +6 more
TL;DR: In this article, a biomimetic nanofluidic diode whose ionic transport characteristics can be completely modulated with the proton concentration in solution is demonstrated, where the fabrication procedure involves the electrostatic assembly of poly(allylamine hydrochloride) (PAH) into a track-etched conical nanochannel.
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TL;DR: A public database of common variation in the human genome: more than one million single nucleotide polymorphisms for which accurate and complete genotypes have been obtained in 269 DNA samples from four populations, including ten 500-kilobase regions in which essentially all information about common DNA variation has been extracted.
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