Journal ArticleDOI
Nanopore sensors for nucleic acid analysis
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TLDR
This article reviews the use of nanopore technology in DNA sequencing, genetics and medical diagnostics and suggests that nanopore-based sensors could be competitive with other third-generation DNA sequencing technologies.Abstract:
Nanopore analysis is an emerging technique that involves using a voltage to drive molecules through a nanoscale pore in a membrane between two electrolytes, and monitoring how the ionic current through the nanopore changes as single molecules pass through it. This approach allows charged polymers (including single-stranded DNA, double-stranded DNA and RNA) to be analysed with subnanometre resolution and without the need for labels or amplification. Recent advances suggest that nanopore-based sensors could be competitive with other third-generation DNA sequencing technologies, and may be able to rapidly and reliably sequence the human genome for under $1,000. In this article we review the use of nanopore technology in DNA sequencing, genetics and medical diagnostics.read more
Citations
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Journal ArticleDOI
A Patch-Clamp ASIC for Nanopore-Based DNA Analysis
TL;DR: A fully integrated high-sensitivity patch-clamp system is proposed for single-molecule deoxyribonucleic acid (DNA) analysis using a nanopore sensor and will functionalize single and multiple solid-state nanopores formed in integrated microfluidic devices for advanced DNA analysis, in scientific and diagnostic applications.
Journal ArticleDOI
Ion transport through a graphene nanopore
Guohui Hu,Mao Mao,Sandip Ghosal +2 more
TL;DR: Molecular dynamics simulation is utilized to investigate the ionic transport of NaCl in solution through a graphene nanopore under an applied electric field and results show the formation of concentration polarization layers in the vicinity of the graphene sheet.
Journal ArticleDOI
Nanobiosensors in diagnostics
TL;DR: Biosensors (simple, robust, sensitive, cost-effective) combined with nanomaterials, also called nanobiosensor, are serving as bridge between advanced detection/diagnostics and daily/routine tests.
Journal ArticleDOI
DNA nanostructure-based ultrasensitive electrochemical microRNA biosensor.
Yanli Wen,Gang Liu,Hao（裴浩） Pei,Li Lanying,Xu Qin,Liang Wen,Yan Li,Li Xu,Suzhen Ren,Chunhai（樊春海） Fan +9 more
TL;DR: Since this ultrasensitive electrochemical miRNA sensor (EMRS) is highly reproducible and essentially free of prior target labeling and PCR amplification, it can conveniently and reliably analyze miRNA expression levels in clinical samples from esophageal squamous cell carcinoma patients.
Journal ArticleDOI
Fabrication and characterization of nanopores with insulated transverse nanoelectrodes for DNA sensing in salt solution.
TL;DR: The fabrication, simulation, and characterization of insulated nanoelectrodes aligned with nanopores in low‐capacitance silicon nitride membrane chips are reported on, exploring these devices for the transverse sensing of DNA molecules as they are electrophoretically driven through the nanopore in a linear fashion.
References
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TL;DR: A public database of common variation in the human genome: more than one million single nucleotide polymorphisms for which accurate and complete genotypes have been obtained in 269 DNA samples from four populations, including ten 500-kilobase regions in which essentially all information about common DNA variation has been extracted.
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