Journal ArticleDOI
Nanopore sensors for nucleic acid analysis
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TLDR
This article reviews the use of nanopore technology in DNA sequencing, genetics and medical diagnostics and suggests that nanopore-based sensors could be competitive with other third-generation DNA sequencing technologies.Abstract:
Nanopore analysis is an emerging technique that involves using a voltage to drive molecules through a nanoscale pore in a membrane between two electrolytes, and monitoring how the ionic current through the nanopore changes as single molecules pass through it. This approach allows charged polymers (including single-stranded DNA, double-stranded DNA and RNA) to be analysed with subnanometre resolution and without the need for labels or amplification. Recent advances suggest that nanopore-based sensors could be competitive with other third-generation DNA sequencing technologies, and may be able to rapidly and reliably sequence the human genome for under $1,000. In this article we review the use of nanopore technology in DNA sequencing, genetics and medical diagnostics.read more
Citations
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Journal ArticleDOI
The Nanopore-Tweezing-Based, Targeted Detection of Nucleobases on Short Functionalized Peptide Nucleic Acid Sequences.
Isabela S. Dragomir,Alina Asandei,Irina Schiopu,Ioana C. Bucataru,Loredana Mereuta,Tudor Luchian +5 more
TL;DR: In this article, the authors employed the nanopore tweezing technique to capture amino acid-functionalized peptide nucleic acids (PNAs) with α-hemolysin-based nanopores and correlated the ensuing stochastic fluctuations of the ionic current through nanopore with the composition and order of bases in the PNAs primary structure.
Book ChapterDOI
Synthesis, Characterization, and Applications of Nanoporous Materials for Sensing and Separation
Culture-independent methods and high-throughput sequencing applied to evolutionary microbial genomics
TL;DR: Culture-independent methods allow us to better understand the diversity of microorganisms on earth by omitting the culturing step, and gain access to species previously out of the authors' reach.
Journal ArticleDOI
Grain boundary-assisted resistance to crack propagation in nanoporous gold with fine grains
TL;DR: In this paper , a cyclic hot-rolling is adopted to prepare fine grain structure in Au-Ag precursor alloys, which evolve to fine grained np-Au with grain size of 3.69 µm after dealloying.
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TL;DR: A public database of common variation in the human genome: more than one million single nucleotide polymorphisms for which accurate and complete genotypes have been obtained in 269 DNA samples from four populations, including ten 500-kilobase regions in which essentially all information about common DNA variation has been extracted.
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