Journal ArticleDOI
Nanopore sensors for nucleic acid analysis
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TLDR
This article reviews the use of nanopore technology in DNA sequencing, genetics and medical diagnostics and suggests that nanopore-based sensors could be competitive with other third-generation DNA sequencing technologies.Abstract:
Nanopore analysis is an emerging technique that involves using a voltage to drive molecules through a nanoscale pore in a membrane between two electrolytes, and monitoring how the ionic current through the nanopore changes as single molecules pass through it. This approach allows charged polymers (including single-stranded DNA, double-stranded DNA and RNA) to be analysed with subnanometre resolution and without the need for labels or amplification. Recent advances suggest that nanopore-based sensors could be competitive with other third-generation DNA sequencing technologies, and may be able to rapidly and reliably sequence the human genome for under $1,000. In this article we review the use of nanopore technology in DNA sequencing, genetics and medical diagnostics.read more
Citations
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Journal ArticleDOI
Changes in dynamical behavior of ionic liquid in silica nano-pores
TL;DR: In this article, two dielectric relaxation peaks have been observed in the confined ionic liquid (IL) while there is only one relaxation peak for bulk IL and the two relaxation peaks are assigned to the different dynamical behaviors of the central core and layered IL molecules.
Journal ArticleDOI
First-principles versus semi-empirical modeling of global and local electronic transport properties of graphene nanopore-based sensors for DNA sequencing
TL;DR: In this paper, the authors examined changes in the total and local electronic currents within the plane of graphene nanoribbon with zigzag edges (ZGNR) hosting a nanopore which are induced by inserting a DNA nucleobase into the pore.
Journal ArticleDOI
Identification of four single-stranded DNA homopolymers with a solid-state nanopore in alkaline CsCl solution.
TL;DR: An alkaline CsCl aqueous solution is introduced, which denatures the G-quadruplex into a single-stranded structure by disrupting the hydrogen-bonding network between the guanines and preventing the binding of the K+ ion to G-quartets, and provided a proof-of-principle that single-Stranded DNA homopolymers of all four nucleotides could be statistically identified according to their blockade currents with the same single nanopore.
Journal ArticleDOI
Polymer translocation into and out of an ellipsoidal cavity
TL;DR: It is found that both polymer ejection and insertion are faster for ellipsoidal cavities than for spherical cavities, and the discrepancy is likely due to out-of-equilibrium conformational behaviour that is not accounted for in the FP approach.
Journal ArticleDOI
Nanoscale Semiconductor Devices as New Biomaterials.
TL;DR: Why it is necessary to build interfaces between cells and semiconductor nanoelectronics and the use of nanowire transistors as electrical recording devices that can be integrated into synthetic tissues and targeted intra- or extracellularly to study single cells are discussed.
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TL;DR: A public database of common variation in the human genome: more than one million single nucleotide polymorphisms for which accurate and complete genotypes have been obtained in 269 DNA samples from four populations, including ten 500-kilobase regions in which essentially all information about common DNA variation has been extracted.
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