Journal ArticleDOI
Nanopore sensors for nucleic acid analysis
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TLDR
This article reviews the use of nanopore technology in DNA sequencing, genetics and medical diagnostics and suggests that nanopore-based sensors could be competitive with other third-generation DNA sequencing technologies.Abstract:
Nanopore analysis is an emerging technique that involves using a voltage to drive molecules through a nanoscale pore in a membrane between two electrolytes, and monitoring how the ionic current through the nanopore changes as single molecules pass through it. This approach allows charged polymers (including single-stranded DNA, double-stranded DNA and RNA) to be analysed with subnanometre resolution and without the need for labels or amplification. Recent advances suggest that nanopore-based sensors could be competitive with other third-generation DNA sequencing technologies, and may be able to rapidly and reliably sequence the human genome for under $1,000. In this article we review the use of nanopore technology in DNA sequencing, genetics and medical diagnostics.read more
Citations
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Journal ArticleDOI
Electronic signature of DNA bases via Z-shaped graphene nanoribbon with a nanopore
TL;DR: In this article, a two-terminal z-shaped sensor is developed and studied to detect the sequence of DNA nucleobases, which consists of two metallic zigzag graphene nanoribbon (ZGNR) and a semiconducting channel made of armchair graphene nanorsoribbons (AGNR), and a nanopore in the middle of the channel through which DNA nucleabases are translocated.
Journal ArticleDOI
Endohedral confinement of a DNA dodecamer onto pristine carbon nanotubes and the stability of the canonical B form
TL;DR: Using enhanced sampling techniques, it is shown for the first time that, given a sufficiently large carbon nanotube, the confinement of a double-stranded DNA segment, 5'-D(*CP*GP*CP* GP* AP*AP*TP*TP-CP*CP-GP*G)-3', is thermodynamically favourable under physiological environments, leading to DNA-nanotube hybrids with lower free energy than the unconfined biomolecule.
Journal ArticleDOI
Nanopore Current Oscillations: Nonlinear Dynamics on the Nanoscale.
TL;DR: This Letter considers the description of voltage-induced current fluctuations through a single nanopore from the perspective of nonlinear dynamics, and presents a simple phenomenological nonlinear model that reproduces the qualitative behavior of the experimental data.
Journal ArticleDOI
Graphene nanopore support system for simultaneous high-resolution AFM imaging and conductance measurements
TL;DR: A graphene-based single-nanopore support is designed and integrated AFM imaging and ionic conductance recording is integrated using a newly designed double-chamber recording system to study an overlaid thin film.
Journal ArticleDOI
Differentiating Metastatic and Non-metastatic Tumor Cells from Their Translocation Profile through Solid-State Micropores
Waqas Ali,Azhar Ilyas,Azhar Ilyas,Loan Bui,Bailey Sayles,Yeun Hur,Young Tae Kim,Samir M. Iqbal +7 more
TL;DR: The micropore approach is simple, exact, and quantitative for metastatic cell detection in a lab-on-a chip setting, without the need for any preprocessing of the sample.
References
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TL;DR: A public database of common variation in the human genome: more than one million single nucleotide polymorphisms for which accurate and complete genotypes have been obtained in 269 DNA samples from four populations, including ten 500-kilobase regions in which essentially all information about common DNA variation has been extracted.
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