Journal ArticleDOI
Nanopore sensors for nucleic acid analysis
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TLDR
This article reviews the use of nanopore technology in DNA sequencing, genetics and medical diagnostics and suggests that nanopore-based sensors could be competitive with other third-generation DNA sequencing technologies.Abstract:
Nanopore analysis is an emerging technique that involves using a voltage to drive molecules through a nanoscale pore in a membrane between two electrolytes, and monitoring how the ionic current through the nanopore changes as single molecules pass through it. This approach allows charged polymers (including single-stranded DNA, double-stranded DNA and RNA) to be analysed with subnanometre resolution and without the need for labels or amplification. Recent advances suggest that nanopore-based sensors could be competitive with other third-generation DNA sequencing technologies, and may be able to rapidly and reliably sequence the human genome for under $1,000. In this article we review the use of nanopore technology in DNA sequencing, genetics and medical diagnostics.read more
Citations
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Journal ArticleDOI
Recent Progress in Solid-State Nanopores.
TL;DR: The fundamental principles of the solid-state nanopore are summarized to illustrate the novelty of the device, and improvements in the performance of the platform in terms of device fabrication are explained.
Journal ArticleDOI
High-throughput optical sensing of nucleic acids in a nanopore array
Shuo Huang,Mercedes Romero-Ruiz,Oliver Kieran Castell,Oliver Kieran Castell,Hagan Bayley,Mark I. Wallace +5 more
TL;DR: By optically encoding the ionic flux through protein nanopores, the discrimination of nucleic Acid sequences and the detection of sequence-specific nucleic acid binding events can be parallelized and high-throughput nanopore recording is shown.
Journal ArticleDOI
High spatial resolution nanoslit SERS for single-molecule nucleobase sensing.
Chang Chen,Yi Li,Sarp Kerman,Pieter Neutens,Kherim Willems,Sven Cornelissen,Liesbet Lagae,Tim Stakenborg,Pol Van Dorpe +8 more
TL;DR: This work uses an optically engineered elongated nanopore structure, a plasmonic nanoslit, to locally enable single-molecule surface enhanced Raman spectroscopy (SERS) for identifying nucleobases, both individual and incorporated in DNA strands.
Journal ArticleDOI
Two-dimensional material membranes: an emerging platform for controllable mass transport applications.
TL;DR: In this review, the latest applications based on high impermeability and selective permeation of these 2D material membranes are overviewed for different fields, including environmental control, chemical engineering, electronic devices, and biosensors.
Journal ArticleDOI
Silicene as a new ultrafast DNA sequencing device
TL;DR: In this article, the authors investigated the stability of a nano-bio system, its electronic properties and the pronounced effects on the transverse electronic transport, i.e., changes in the transmission and the conductance caused by adsorption of each nucleobase, explored by them through the nonequilibrium Green's function method.
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TL;DR: A public database of common variation in the human genome: more than one million single nucleotide polymorphisms for which accurate and complete genotypes have been obtained in 269 DNA samples from four populations, including ten 500-kilobase regions in which essentially all information about common DNA variation has been extracted.
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