Journal ArticleDOI
Nanopore sensors for nucleic acid analysis
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TLDR
This article reviews the use of nanopore technology in DNA sequencing, genetics and medical diagnostics and suggests that nanopore-based sensors could be competitive with other third-generation DNA sequencing technologies.Abstract:
Nanopore analysis is an emerging technique that involves using a voltage to drive molecules through a nanoscale pore in a membrane between two electrolytes, and monitoring how the ionic current through the nanopore changes as single molecules pass through it. This approach allows charged polymers (including single-stranded DNA, double-stranded DNA and RNA) to be analysed with subnanometre resolution and without the need for labels or amplification. Recent advances suggest that nanopore-based sensors could be competitive with other third-generation DNA sequencing technologies, and may be able to rapidly and reliably sequence the human genome for under $1,000. In this article we review the use of nanopore technology in DNA sequencing, genetics and medical diagnostics.read more
Citations
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A microfluidic streaming potential analyzer for label-free DNA detection
TL;DR: The microfluidic streaming potential analyzer functionalized with PNA probes provides a simple, inexpensive and reusable platform for nucleic acid detection.
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Stretching of Tethered DNA in Nanoslits
TL;DR: This work measures the force–extension relation of tethered DNA and describes this relation with modified wormlike chain models and allows experimental validations of several theoretical predictions, including the increase in the global persistence length of confined DNA with increasing degree of confinement and the “confined Pincus” regime in slit confinement.
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A coarse-grained DNA model for the prediction of current signals in DNA translocation experiments
TL;DR: This work presents an implicit solvent coarse-grained double-stranded DNA model confined to an infinite cylindrical pore that reproduces the experimentally observed current modulations of a KaCl solution at various concentrations and achieves a consistent description of the system's electrokinetics.
Journal ArticleDOI
RNA nanopatterning on graphene
Qiang Li,Qiang Li,Jens P. Froning,Martin Pykal,Shuai Zhang,Z Wang,M Vondrák,Pavel Banáš,Klára Čépe,Petr Jurečka,Jiří Šponer,Radek Zbořil,Mingdong Dong,Michal Otyepka +13 more
TL;DR: In this article, an RNA hairpin was used as a model system to understand the structural and morphological organization of biomolecules on graphene in water, and the key parameters governing the RNA's behavior on the graphene surface were the number of graphene layers, RNA concentration, and temperature.
Journal ArticleDOI
Controlled transport of DNA through a Y-shaped carbon nanotube in a solid membrane.
TL;DR: It is found that the dsDNA molecule can be unzipped at the junction of the Y-CNT by applying constant or alternating biasing voltages.
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TL;DR: A public database of common variation in the human genome: more than one million single nucleotide polymorphisms for which accurate and complete genotypes have been obtained in 269 DNA samples from four populations, including ten 500-kilobase regions in which essentially all information about common DNA variation has been extracted.
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