Journal ArticleDOI
Nanopore sensors for nucleic acid analysis
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TLDR
This article reviews the use of nanopore technology in DNA sequencing, genetics and medical diagnostics and suggests that nanopore-based sensors could be competitive with other third-generation DNA sequencing technologies.Abstract:
Nanopore analysis is an emerging technique that involves using a voltage to drive molecules through a nanoscale pore in a membrane between two electrolytes, and monitoring how the ionic current through the nanopore changes as single molecules pass through it. This approach allows charged polymers (including single-stranded DNA, double-stranded DNA and RNA) to be analysed with subnanometre resolution and without the need for labels or amplification. Recent advances suggest that nanopore-based sensors could be competitive with other third-generation DNA sequencing technologies, and may be able to rapidly and reliably sequence the human genome for under $1,000. In this article we review the use of nanopore technology in DNA sequencing, genetics and medical diagnostics.read more
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Electrical Chips for Biological Point-of-Care Detection
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Electrically Tunable Quenching of DNA Fluctuations in Biased Solid-State Nanopores
TL;DR: This study investigates how in the case of a double-stranded DNA the single-molecule sensitivity can be improved through bias voltages, and opens a feasible and efficient route to reduce thermal noise and, in turn, enhance the signal-to-noise ratio in single-Molecule nanopore sensing.
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Nanoporentechniken zur Sequenzierung und Detektion von Nukleinsäuren
TL;DR: In this paper, a verfahren erlaubt die selektive analyse von Nukleinsauren in Echtzeit und im Hochdurchsatz.
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Direct Analysis of Incorporation of an Anticancer Drug into DNA at Single-Molecule Resolution.
Takahito Ohshiro,Yuuki Komoto,Masamitsu Konno,Jun Koseki,Ayumu Asai,Hideshi Ishii,Masateru Taniguchi +6 more
TL;DR: A means to detect FTD in a single-stranded oligonucleotide using a method to distinguish single molecules by differences in electrical conductance is reported, the first direct observation of its kind.
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Bio-inspired Track-Etched Polymeric Nanochannels: Steady-State Biosensors for Detection of Analytes.
TL;DR: In this paper, the authors summarized the recent advances in bio-inspired polymeric nanochannels as sensing platforms for detection of important analytes in living organisms, to meet the high demand for high-performance biosensors for analysis of target analytes, and the potential for development of smart sensing devices.
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TL;DR: A public database of common variation in the human genome: more than one million single nucleotide polymorphisms for which accurate and complete genotypes have been obtained in 269 DNA samples from four populations, including ten 500-kilobase regions in which essentially all information about common DNA variation has been extracted.
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