RT-LAMP CRISPR-Cas12/13-Based SARS-CoV-2 Detection Methods.
Kasturi Selvam,Mohamad Ahmad Najib,Muhammad Fazli Khalid,Suharni Mohamad,Fahreddin Palaz,Mehmet Ozsoz,Ismail Aziah +6 more
- Vol. 11, Iss: 9, pp 1646
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The article was published on 2021-09-08 and is currently open access. It has received 18 citations till now.read more
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Use of MALDI-TOF mass spectrometry for virus identification: a review.
TL;DR: This review focuses on matrix-assisted laser desorption and ionisation with time-of-flight analyser mass spectrometry (MALDI-TOF MS) techniques as potential tools for the diagnosis of viruses with an emphasis on SARS-CoV-2.
Journal ArticleDOI
LAMP-Based Point-of-Care Biosensors for Rapid Pathogen Detection
TL;DR: In this article , the advances in LAMP-based point-of-care diagnostics assays developed during the past few years for rapid and sensitive detection of infectious pathogens are outlined.
Journal ArticleDOI
Paper-Based Biosensors for the Detection of Nucleic Acids from Pathogens
Jiangshan Wang,Josiah Levi Davidson,Simerdeep Kaur,A. Dextre,Mohsen Ranjbaran,Mohamed S. Kamel,Shreya Milind Athalye,Mohit S. Verma +7 more
TL;DR: Paper-based biosensors are microfluidic analytical devices used for the detection of biochemical substances, such as nucleic acid-based pathogen detection and point-of-care testing as mentioned in this paper .
Journal ArticleDOI
Investigation and validation of labelling loop mediated isothermal amplification (LAMP) products with different nucleotide modifications for various downstream analysis
TL;DR: In this article , the effects on amplification and labeling efficiency were studied as a function of molecule size and nucleotide amount as well as target concentration, and it was shown that diverse labelling of LAMP amplicons can be achieved using different, modified nucleotides during LAMP and that these samples can be analysed by a wide range of downstream applications such as fluorescence spectroscopy, gel electrophoresis, microarrays and lateral flow systems.
Journal ArticleDOI
An improved, simple and field‐deployable CRISPR‐Cas12a assay for the detection of SARS‐CoV‐2
Chitra Seetharam Misra,Shyam Sunder Rangu,Ravindra D Phulsundar,Gargi Bindal,Sandeep Singh,Ravindranath Shashidhar,T. K. Saha,Avss Narayana Rao,Devashish Rath +8 more
TL;DR: The main goal of this study was to improve the Cas12a‐based SARS‐CoV‐2 DETECTR method and develop a portable and field‐deployable system to reduce the recurring consumable cost.
References
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Peng Zhou,Xing-Lou Yang,Xian Guang Wang,Ben Hu,Lei Zhang,Wei Zhang,Hao Rui Si,Yan Zhu,Bei Li,Chao Lin Huang,Hui-Dong Chen,Jing Chen,Yun Luo,Hua Guo,Ren Di Jiang,Meiqin Liu,Ying Chen,Xu Rui Shen,Xi Wang,Xiao Shuang Zheng,Kai Zhao,Quanjiao Chen,Fei Deng,Lin Lin Liu,Bing Yan,Fa Xian Zhan,Yan-Yi Wang,Gengfu Xiao,Zhengli Shi +28 more
TL;DR: Identification and characterization of a new coronavirus (2019-nCoV), which caused an epidemic of acute respiratory syndrome in humans in Wuhan, China, and it is shown that this virus belongs to the species of SARSr-CoV, indicates that the virus is related to a bat coronav virus.
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Structure, Function, and Antigenicity of the SARS-CoV-2 Spike Glycoprotein.
Alexandra C. Walls,Young-Jun Park,M. Alejandra Tortorici,M. Alejandra Tortorici,Abigail Wall,Andrew T. McGuire,Andrew T. McGuire,David Veesler +7 more
TL;DR: It is demonstrating that cross-neutralizing antibodies targeting conserved S epitopes can be elicited upon vaccination, and it is shown that SARS-CoV-2 S uses ACE2 to enter cells and that the receptor-binding domains of Sars- coV- 2 S and SARS S bind with similar affinities to human ACE2, correlating with the efficient spread of SATS among humans.
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Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR.
Victor M. Corman,Olfert Landt,Marco Kaiser,Richard Molenkamp,Adam Meijer,Daniel K.W. Chu,Tobias Bleicker,Sebastian Brünink,Julia Schneider,Marie Luisa Schmidt,Daphne G.J.C. Mulders,Bart L. Haagmans,Bas van der Veer,Sharon van den Brink,Lisa Wijsman,Gabriel Goderski,Jean Louis Romette,Joanna Ellis,Maria Zambon,Malik Peiris,Herman Goossens,Chantal B.E.M. Reusken,Marion Koopmans,Christian Drosten +23 more
TL;DR: A validated diagnostic workflow for 2019-nCoV is presented, its design relying on close genetic relatedness of 2019- nCoV with SARS coronavirus, making use of synthetic nucleic acid technology.
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CRISPR provides acquired resistance against viruses in prokaryotes
Rodolphe Barrangou,Christophe Fremaux,Hélène Deveau,Melissa Richards,Patrick Boyaval,Sylvain Moineau,Dennis A. Romero,Philippe Horvath +7 more
TL;DR: It is found that, after viral challenge, bacteria integrated new spacers derived from phage genomic sequences, and CRISPR provided resistance against phages, and resistance specificity is determined by spacer-phage sequence similarity.
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Origin and evolution of pathogenic coronaviruses
Jie Cui,Fang Li,Zhengli Shi +2 more
TL;DR: The viral factors that enabled the emergence of diseases such as severe acute respiratory syndrome and Middle East respiratory syndrome are explored and the diversity and potential of bat-borne coronaviruses are highlighted.
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