Book ChapterDOI
Selected methods for the determination of ascorbic acid in animal cells, tissues, and fluids
TLDR
This chapter discusses selected methods for the determination of ascorbic acid in animal cells, tissues, and fluids and suggests that prompt stabilization is especially important in the case of plasma or serum.Abstract:
Publisher Summary This chapter discusses selected methods for the determination of ascorbic acid in animal cells, tissues, and fluids. Methods for determining ascorbic acid are numerous. In general, chemical analyses for the vitamin are divided into two groups; the determination of the reduced form and the determination of the oxidized form. The former group of analyses is usually based upon the oxidation–reduction properties of ascorbic acid. These are widely used as the fundamental reactions in the measurement of vitamin C. The latter group of analyses is usually based upon the oxidation of the ascorbic acid and the subsequent formation of a hydrazone or a fluorophore. Best results are obtained if samples, especially plasma, are quickly stabilized with either trichloroacetic acid or metaphosphoric acid and immediately analyzed. Prompt stabilization is especially important in the case of plasma or serum. The greater stability of ascorbic acid in acid solution is because of the decreased tendency for the hydrolysis of the lactone ring with decreasing pH.read more
Citations
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Journal ArticleDOI
Cardioprotective effect of aqueous, ethanol and aqueous ethanol extract of Aerva lanata (Linn.) against doxorubicin induced cardiomyopathy in rats
Paramasivam Ragavendran,Dominic Sophia,Chinthamony Arulraj,Velliyur Kanniappan Gopalakrishnan +3 more
TL;DR: The cardioprotective effect of aqueous, ethanol and aqueously ethanol extract of Aerva lanata (A. lanata) against doxorubicin induced cardiomyopathy in Wistar rats is evaluated.
Journal ArticleDOI
Chinese bayberry fruit extract alleviates oxidative stress and prevents 1,2-dimethylhydrazine-induced aberrant crypt foci development in rat colon carcinogenesis
TL;DR: Results indicate that CBFE, rich in phenolic compounds, effectively inhibits DMH-induced ACF and colonic tumour development by alleviating oxidative stress.
Journal ArticleDOI
Quercetin in combination with vitamins (C and E) improve oxidative stress and hepatic injury in cadmium intoxicated rats
TL;DR: The ameliorative effect against Cd intoxication was much pronounced in rats treated with QE along with vitamins C and E and the histo-pathological changes when compared to normal control rats.
Journal ArticleDOI
Changes in oxidative status in periparturient dairy cows in hot conditions
TL;DR: In this article, the authors investigated the changes in the plasma concentrations of oxidative stress markers in periparturient dairy cows in hot conditions and found that oxidative stress increased in cows after parturition under hot climatic conditions.
Journal ArticleDOI
Counteracting adriamycin-induced oxidative stress by administration of N-acetyl cysteine and vitamin E.
Periandavan Kalaiselvi,Viswanathan Pragasam,Srinivasan Chinnikrishnan,Coothan Kandaswamy Veena,Rajaguru Sundarapandiyan,Palaninathan Varalakshmi +5 more
TL;DR: Treatment with NAC and vitamin E 1day prior to ADR administration significantly reduced lipid peroxidation and prevented the necrosis caused by ADR, thereby proving to be an effective thiol replenishing agent and antioxidant.
References
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Journal ArticleDOI
The determination of ascorbic acid in whole blood and urine through the 2,4-dinitrophenylhydrazine derivative of dehydroascorbic acid
Joseph H. Roe,Carl A. Kuether +1 more
Journal ArticleDOI
Distribution of ascorbic acid, metabolites and analogues in man and animals.
TL;DR: The availability of labeled AA, metabolites, and analogues has made it possible to follow up the appearance of these compounds or mctabolites thereof in various tissues of animals by means of dissecting the animals with subsequent determination of the radioactive material accumulated by the tissues or by Means of whole-body autoradiography.
Journal ArticleDOI
A rapid micromethod for the determination of ascorbic acid in plasma and tissues.
TL;DR: A rapid simple micromethod for the determination of l -ascorbic acid in plasma and other biological tissues using orthophosphoric acid and ferric iron is presented and can be used to accurately determine 0.1 μg of the vitamin in samples of plasma andOther biological tissues.